Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Brain Res. 2010 Nov 18;1361:31-42. doi: 10.1016/j.brainres.2010.09.045. Epub 2010 Sep 19.
Physical exercise is known to enhance brain function in several aspects. We evaluated the acute effects of a moderate forced exercise protocol on synaptic proteins, namely synapsin I (SYN) and synaptophysin (SYP), and structural proteins (neurofilaments, NFs) in rat brain regions related to motor function and often affected by neurodegenerative disorders. Immunohistochemistry, Western blotting and real-time PCR were used to analyze the expression of those proteins after 3, 7 and 15days of exercise (EX3, EX7 and EX15). In the cerebellum, increase of SYN was observed at EX7 and EX15 and of NF68 at EX3. In the substantia nigra, increases of protein levels were observed for NF68 and NF160 at EX3. In the striatum, there was an increase of SYN at EX3 and EX7, of SYP at EX7 and of NF68 at EX3. In the cortex, decreased levels of NF68 and NF160 were observed at EX3, followed by an increase of NF68 at EX15. In the reticular formation, all NF proteins were increased at EX15. The mRNA data for each time-point and region also revealed significant exercise-related changes of SYN, SYP and NF expression. These results suggest that moderate physical exercise modulates synaptic and structural proteins in motor brain areas, which may play an important role in the exercise-dependent brain plasticity.
体育锻炼在多个方面被证明能增强大脑功能。我们评估了中等强度强制运动方案对与运动功能相关且常受神经退行性疾病影响的脑区中突触蛋白（突触素 I（SYN）和突触小体蛋白（SYP））和结构蛋白（神经丝，NFs）的急性影响。免疫组织化学、Western blot 和实时 PCR 用于分析运动后 3、7 和 15 天（EX3、EX7 和 EX15）这些蛋白的表达。在小脑，SYN 在 EX7 和 EX15 时增加，NF68 在 EX3 时增加。在黑质，NF68 和 NF160 的蛋白水平在 EX3 时增加。在纹状体，SYN 在 EX3 和 EX7 时增加，SYP 在 EX7 时增加，NF68 在 EX3 时增加。在皮质，NF68 和 NF160 的水平在 EX3 时降低，随后在 EX15 时增加。在网状结构，所有 NF 蛋白在 EX15 时增加。每个时间点和区域的 mRNA 数据也显示了 SYN、SYP 和 NF 表达的显著运动相关变化。这些结果表明，中等强度的体育锻炼调节运动脑区的突触和结构蛋白，这可能在运动依赖的大脑可塑性中发挥重要作用。
