Department of Comparative Pathbiology, Purdue University, West Lafayette, IN 47907, USA.
Vet Pathol. 2011 Mar;48(2):443-50. doi: 10.1177/0300985810382095. Epub 2010 Sep 21.
The immunoreactivity of PNL2 and antityrosinase in formalin-fixed, paraffin-embedded canine melanocytic neoplasms (n = 101) was compared with that of Melan A. Of the 113 samples overall, 106 were positive for PNL2, 101 for Melan A, and 90 for tyrosinase. Six melanomas that were positive for PNL2 were negative for Melan A; 1 melanoma that was negative for PNL2 was positive for Melan A. Eighty tumors were positive for all 3 markers; 111 reacted with at least 1 the 3 antibodies. Decalcification with formic acid for up to 1 week did not affect immunoreactivity of any of the markers; however, decalcification with HCl for 1 day or 1 week notably decreased or completely abrogated immunoreactivity for Melan A and PNL2. There was only minor loss of immunoreactivity for tyrosinase in tissues decalcified with HCl for 1 week. Prolonged fixation (up to 2 months) did not affect PNL2 or tyrosinase immunoreactivity; however, Melan A immunoreactivity was reduced after 1 month of fixation. PNL2 was not expressed in 120 nonmelanocytic tumors (carcinomas, sarcomas, steroid-producing tumors, and leukocytic tumors). In summary, antibody PNL2 is slightly more sensitive than Melan A and more sensitive than tyrosinase in the identification of canine melanocytic neoplasms. Furthermore, PNL2 does not appear to cross-react with nonmelanocytic neoplasms. PNL2 is resistant to prolonged fixation but sensitive to strong decalcification. Results indicate that PNL2 is an excellent marker in the identification of canine melanomas and that the sensitivity is close to 100% when used in conjunction with Melan A and tyrosinase.
将 PNL2 和抗酪氨酸酶在福尔马林固定、石蜡包埋的犬黑色素瘤肿瘤（n = 101）中的免疫反应性与 Melan A 进行了比较。在总共 113 个样本中，106 个对 PNL2 呈阳性，101 个对 Melan A 呈阳性，90 个对酪氨酸酶呈阳性。6 个对 PNL2 呈阳性的黑色素瘤对 Melan A 呈阴性；1 个对 PNL2 呈阴性的黑色素瘤对 Melan A 呈阳性。80 个肿瘤对所有 3 种标志物均呈阳性；111 个反应至少 1 种抗体。用甲酸脱钙长达 1 周不会影响任何标志物的免疫反应性；然而，用 HCl 脱钙 1 天或 1 周会显著降低或完全消除 Melan A 和 PNL2 的免疫反应性。用 HCl 脱钙 1 周对酪氨酸酶的免疫反应性仅有轻微损失。长时间固定（长达 2 个月）不会影响 PNL2 或酪氨酸酶的免疫反应性；然而，固定 1 个月后，Melan A 的免疫反应性降低。PNL2 在 120 个非黑色素瘤肿瘤（癌、肉瘤、类固醇产生肿瘤和白细胞肿瘤）中不表达。总之，抗体 PNL2 在鉴定犬黑色素瘤方面比 Melan A 略敏感，比酪氨酸酶更敏感。此外，PNL2 似乎与非黑色素瘤肿瘤没有交叉反应。PNL2 耐长时间固定但对强烈脱钙敏感。结果表明，PNL2 是鉴定犬黑色素瘤的一个极好的标志物，当与 Melan A 和酪氨酸酶联合使用时，其敏感性接近 100%。
