Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, AZ 85721-0038, USA.
Anal Bioanal Chem. 2010 Nov;398(6):2693-700. doi: 10.1007/s00216-010-4201-y. Epub 2010 Sep 22.
This work presents the use of integrated, liquid core, optical waveguides for measuring immunoagglutination-induced light scattering in a microfluidic device, towards rapid and sensitive detection of avian influenza (AI) viral antigens in a real biological matrix (chicken feces). Mie scattering simulations were performed and tested to optimize the scattering efficiency of the device through proper scatter angle waveguide geometry. The detection limit is demonstrated to be 1 pg mL(-1) in both clean buffer and real biological matrix. This low detection limit is made possible through on-chip diffusional mixing of AI target antigens and high acid content microparticle assay reagents, coupled with real-time monitoring of immunoagglutination-induced forward Mie scattering via high refractive index liquid core optical waveguides in close proximity (100 μm) to the sample chamber. The detection time for the assay is <2 min. This device could easily be modified to detect trace levels of any biological molecules that antibodies are available for, moving towards a robust platform for point-of-care disease diagnostics.
本工作展示了在微流控装置中使用集成的、液芯、光波导来测量免疫凝集诱导的光散射，以实现对真实生物基质（鸡粪便）中的禽流感（AI）病毒抗原的快速和灵敏检测。进行了 Mie 散射模拟，并通过适当的散射角波导几何形状来测试优化器件的散射效率。在清洁缓冲液和真实生物基质中，检测限均证明低至 1 pg mL(-1)。通过在芯片上进行 AI 目标抗原的扩散混合以及高酸度微球分析试剂，再加上通过高折射率液芯光波导实时监测靠近（100 μm）样品腔的免疫凝集诱导的正向 Mie 散射，实现了这种低检测限。该测定的检测时间<2 分钟。该装置可以轻松修改以检测任何具有抗体的生物分子的痕量水平，朝着用于即时护理疾病诊断的稳健平台迈进。
