Department of Biomedical Engineering, The University of Arizona, Tucson, AZ 75721, USA.
Biosensors (Basel). 2023 Aug 15;13(8):820. doi: 10.3390/bios13080820.
Surveillance of viral pathogens in both point-of-care and clinical settings is imperative to preventing the widespread propagation of disease-undetected viral outbreaks can pose dire health risks on a large scale. Thus, portable, accessible, and reliable biosensors are necessary for proactive measures. Polymeric microparticles have recently gained popularity for their size, surface area, and versatility, which make them ideal biosensing tools. This review cataloged recent investigations on polymeric microparticle-based detection platforms across eight virus families. These microparticles were used as labels for detection (often with fluorescent microparticles) and for capturing viruses for isolation or purification (often with magnetic microparticles). We also categorized all methods by the characteristics, materials, conjugated receptors, and size of microparticles. Current approaches were compared, addressing strengths and weaknesses in the context of virus detection. In-depth analyses were conducted for each virus family, categorizing whether the polymeric microparticles were used as labels, for capturing, or both. We also summarized the types of receptors conjugated to polymeric microparticles for each virus family.
在即时护理和临床环境中对病毒病原体进行监测对于防止疾病的广泛传播至关重要——未被发现的病毒爆发可能会对大规模健康造成严重威胁。因此，需要便携式、易于获取且可靠的生物传感器来采取主动措施。聚合物微球因其大小、表面积和多功能性而最近受到关注，使其成为理想的生物传感工具。本综述对基于聚合物微球的八种病毒家族的检测平台的最新研究进行了分类。这些微球被用作检测标记（通常使用荧光微球）和捕获病毒以进行分离或纯化（通常使用磁性微球）。我们还根据微球的特性、材料、共轭受体和大小对所有方法进行了分类。比较了当前的方法，根据病毒检测的上下文讨论了它们的优缺点。我们对每个病毒家族进行了深入分析，将聚合物微球分类为用作标记、用于捕获或两者兼而有之。我们还总结了每种病毒家族与聚合物微球结合的受体类型。
