Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Inorg Chem. 2010 Oct 18;49(20):9113-5. doi: 10.1021/ic101513a.
A xanthene-forming condensation reaction yields rhodol and rhodamine dyes carrying a zinc-binding ligand that includes the aniline-type nitrogen donor of the fluorophores. Upon zinc coordination in neutral aqueous solution, rhodol RF3 behaves as a ratiometric sensor, and rhodamine RA1 acts as a turn-off intensity-based indicator. Both fluorescent compounds bind the divalent zinc cation with micromolar affinity.
一种香豆素形成的缩合反应生成了携带锌结合配体的若丹明和罗丹明染料，该配体包括荧光团的苯胺型氮供体。在中性水溶液中与锌配位时，罗丹明 RF3 表现为比率型传感器，罗丹明 RA1 则作为关闭强度型指示剂。这两种荧光化合物都以微摩尔亲和力与二价锌阳离子结合。
