State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Dalton Trans. 2011 Mar 7;40(9):1984-9. doi: 10.1039/c0dt01262a. Epub 2010 Dec 17.
A novel fluorescent sensor composed of a naphthalene functionalized tetraazamacrocycle ligand 3,6,9,15-tetraazabicyclo[9.3.1]pentadeca-1(15),11,13-triene-3-methyl naphthalene (1) and Zn(2+) has been designed and prepared, which can be utilized for selective and ratiometric sensing of pyrophosphate (PPi) over other phosphate-containing anions in aqueous solution at physiological pH. Notably, the water soluble 1 itself also exhibits a selective enhanced fluorescent response to Zn(2+), and the complex 1-Zn(2+) thus formed eventually fulfils the synergic Zn(2+) coordination-altered strategy with PPi. Furthermore, the ratiometric sensing of 1-Zn(2+) towards PPi performed well even in blood serum milieu. Finally, the sensor 1-Zn(2+) was successfully employed to monitor a real-time assay of inorganic pyrophosphatase (PPase) by means of ratiometric fluorescent measurements for the first time.
一种新型荧光传感器，由萘基功能化的四氮杂大环配体 3,6,9,15-四氮杂双环[9.3.1]十五烷-1(15)，11,13-三烯-3-甲基萘（1）和 Zn(2+)组成，已被设计和制备，可用于在生理 pH 值下在水溶液中选择性和比率检测焦磷酸盐（PPi），而不是其他含磷阴离子。值得注意的是，水溶性 1 本身也对 Zn(2+)表现出选择性增强的荧光响应，而形成的配合物 1-Zn(2+)最终实现了与 PPi 的协同 Zn(2+)配位变化策略。此外，1-Zn(2+)对 PPi 的比率检测在血清环境中也表现良好。最后，该传感器 1-Zn(2+)首次成功用于通过比率荧光测量实时监测无机焦磷酸酶（PPase）的测定。
