Department of Botany, University of Pune, Maharashtra, India.
Appl Biochem Biotechnol. 2011 Mar;163(6):756-64. doi: 10.1007/s12010-010-9080-3. Epub 2010 Sep 24.
Effect of various abiotic (methyl jasmonate, salicylic acid) and biotic (yeast extract, Aspergillus niger) elicitors on furanocoumarin production and in situ product removal was studied using shoot cultures of Ruta graveolens L. Elicitation by yeast extract (1% w/v) on day 15 was most effective. It led to 7.8-fold higher furanocoumarin production that was attained 24 h after elicitation and 43% of the product was released into the medium. Changes in the relative concentration of furanocoumarins produced depend on the elicitor used. Molar ratio of bergapten increased to 93% in response to yeast extract. With the perspective of developing a commercially feasible process, an approach for preserving viability of biomass and its reuse needs to be developed. For this, medium renewal strategy was investigated. Removal of the spent medium 48 h after elicitation allowed in situ product removal and proved effective in revival of cultures, allowing reuse of biomass. A week after medium renewal, the revived biomass was re-elicited and a second furanocoumarin production peak was obtained. A perfusion-based bioprocess optimization approach, employing elicitation coupled with medium renewal with subsequent re-elicitation, as a new strategy for improved furanocoumarin production, has been suggested.
采用芫荽（Ruta graveolens L.）的芽培养物，研究了各种非生物（茉莉酸甲酯、水杨酸）和生物（酵母提取物、黑曲霉）诱导子对呋喃香豆素产生和原位产物去除的影响。在第 15 天用酵母提取物（1%w/v）进行诱导最为有效。这导致呋喃香豆素的产量提高了 7.8 倍，在诱导后 24 小时达到最高水平，其中 43%的产物释放到培养基中。所产生的呋喃香豆素的相对浓度的变化取决于所用的诱导子。对酵母提取物的响应下，佛手柑内酯的摩尔比增加到 93%。从开发可行的商业工艺的角度来看，需要开发一种保持生物量活力并重复使用的方法。为此，研究了培养基更新策略。在诱导后 48 小时去除耗尽的培养基可以实现原位产物去除，并有效地恢复培养物，从而可以重复使用生物量。在培养基更新一周后，重新激活再生生物量，并获得第二次呋喃香豆素生产高峰。建议采用灌流式生物过程优化方法，将诱导与随后的培养基更新相结合，作为提高呋喃香豆素生产的新策略。
