Yamazaki M, Ohye H, Kisugi J, Kamiya H
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Dev Comp Immunol. 1990 Fall;14(4):379-83. doi: 10.1016/0145-305x(90)90030-i.
A bacteriostatic factor, aplysianin P, was purified from the purple fluid of a sea hare, Aplysia kurodai, as a glycoprotein of 60 K daltons. A cytolytic glycoprotein purified from the purple fluid of Dolabella auricularia did not show antibacterial activity. Aplysianin P was half-maximally active for gram-positive and -negative bacteria at 0.2-5.8 micrograms/mL and its action was not bactericidal but bacteriostatic. Aplysianin P completely inhibited the syntheses of DNA and RNA by E. coli, but it did not induce the release of ATP from bacteria. These results suggest that aplysianin P, found in an invertebrate, the sea hare, is a new bacteriostatic protein and that it exerts its action by inhibiting nucleic acid syntheses, as a DNA-inhibiting, chemotherapeutic drug does.
一种抑菌因子——海兔素P，从日本黑兔（Aplysia kurodai）的紫色体液中纯化得到，是一种60千道尔顿的糖蛋白。从耳状芋螺（Dolabella auricularia）的紫色体液中纯化得到的一种溶细胞糖蛋白未显示出抗菌活性。海兔素P对革兰氏阳性菌和阴性菌的半数最大活性浓度为0.2 - 5.8微克/毫升，其作用不是杀菌而是抑菌。海兔素P完全抑制大肠杆菌的DNA和RNA合成，但不诱导细菌释放ATP。这些结果表明，在无脊椎动物海兔中发现的海兔素P是一种新的抑菌蛋白，并且它像一种抑制DNA的化疗药物一样，通过抑制核酸合成发挥作用。
