Yamazaki M, Tansho S, Kisugi J, Muramoto K, Kamiya H
Chem Pharm Bull (Tokyo). 1989 Aug;37(8):2179-82. doi: 10.1248/cpb.37.2179.
A novel cytolytic factor, dolabellanin P, was purified to apparent homogeneity from the purple fluid of the sea hare, Dolabella auricularia. Purified dolabellanin P is a single polypeptide of 60 kDa. The amino acid composition and the N-terminus of the factor were also determined. This factor nonspecifically lysed all the cells tested at 50-200 ng protein/ml. Dolabellanin P caused complete cytolysis within 2 h. The factor is distinct from antineoplastic glycoproteins previously isolated from eggs (aplysianin E) or albumen gland (aplysianin A) of Aplysia kurodai in terms of certain cytolytic properties. These results suggest that dolabellanin P, found in the sea hare, a marine invertebrate, is a new cytolytic factor.
一种新型细胞溶解因子多拉贝兰宁P，从海兔Dolabella auricularia的紫色体液中纯化至表观均一。纯化后的多拉贝兰宁P是一种60 kDa的单一多肽。还测定了该因子的氨基酸组成和N端。该因子在50 - 200 ng蛋白质/毫升时能非特异性裂解所有测试细胞。多拉贝兰宁P在2小时内导致完全细胞溶解。就某些细胞溶解特性而言，该因子与先前从黑鳃海兔的卵（海兔毒素E）或蛋白腺（海兔毒素A）中分离出的抗肿瘤糖蛋白不同。这些结果表明，在海洋无脊椎动物海兔中发现的多拉贝兰宁P是一种新的细胞溶解因子。
