Kisugi J, Ohye H, Kamiya H, Yamazaki M
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Chem Pharm Bull (Tokyo). 1992 Jun;40(6):1537-9. doi: 10.1248/cpb.40.1537.
An antibacterial factor, dolabellanin A, was purified from the albumen gland of a sea hare, Dolabella auricularia. Purified dolabellanin A was a glycoprotein of 250 kilodaltons consisting of 4 subunits, and showed both antibacterial and antineoplastic activities. The two activities were lost in parallel on heating and at low and high pH. This factor was half-maximally active for gram-positive and -negative bacteria at 0.018-0.48 microgram/ml, and its action was not bactericidal but bacteriostatic. Dolabellanin A did not induce morphological elongation of bacteria or the release of adenosine triphosphate, but it completely inhibited the syntheses of deoxyribonucleic acid (DNA) and ribonucleic acid by E. coli within 6 min. These results suggest that dolabellanin A, which is found in a marine invertebrate, the sea hare, is a new antibacterial protein, and that it exerts its action by inhibiting nucleic acid synthesis, as does a DNA-inhibiting chemotherapeutic drug.
一种抗菌因子——耳壳海兔素A，是从海兔（耳壳海兔）的蛋白腺中纯化得到的。纯化后的耳壳海兔素A是一种由4个亚基组成的250千道尔顿的糖蛋白，具有抗菌和抗肿瘤活性。在加热以及低pH和高pH条件下，这两种活性会同时丧失。该因子对革兰氏阳性菌和阴性菌的半最大活性浓度为0.018 - 0.48微克/毫升，其作用不是杀菌而是抑菌。耳壳海兔素A不会诱导细菌形态伸长或三磷酸腺苷的释放，但它能在6分钟内完全抑制大肠杆菌的脱氧核糖核酸（DNA）和核糖核酸的合成。这些结果表明，在海洋无脊椎动物海兔中发现的耳壳海兔素A是一种新型抗菌蛋白，并且它像一种抑制DNA的化疗药物一样，通过抑制核酸合成发挥作用。
