Centre for DNA Nanotechnology, Department of Chemistry and iNANO, Aarhus University, 8000 Århus C, Denmark.
J Am Chem Soc. 2010 Oct 20;132(41):14481-6. doi: 10.1021/ja104456p.
Architectural designs for DNA nanostructures typically fall within one of two broad categories: tile-based designs (assembled from chemically synthesized oligonucleotides) and origami designs (woven structures employing a biological scaffold strand and synthetic staple strands). Both previous designs typically contain many Holliday-type multi-arm junctions. Here we describe the design, implementation, and testing of a unique architectural strategy incorporating some aspects of each of the two previous design categories but without multi-arm junction motifs. Goals for the new design were to use only chemically synthesized DNA, to minimize the number of component strands, and to mimic the back-and-forth, woven strand routing of the origami architectures. The resulting architectural strategy employs "weave tiles" formed from only two oligonucleotides as basic building blocks, thus decreasing the burden of matching multiple strand stoichiometries compared to previous tile-based architectures and resulting in a structurally flexible tile. As an example application, we have shown that the four-helix weave tile can be used to increase the anticoagulant activity of thrombin-binding aptamers in vitro.
DNA 纳米结构的体系结构设计通常属于以下两类之一：基于瓦片的设计（由化学合成的寡核苷酸组装而成）和折纸设计（采用生物支架链和合成钉链的编织结构）。这两种以前的设计通常都包含许多霍利迪型多臂连接。在这里，我们描述了一种独特的体系结构策略的设计、实施和测试，该策略结合了前两类设计的某些方面，但没有多臂连接基序。新设计的目标是仅使用化学合成的 DNA，尽量减少组件链的数量，并模拟折纸结构的来回编织的路线。所得到的体系结构策略采用仅由两个寡核苷酸形成的“编织瓦片”作为基本构建块，从而与以前基于瓦片的体系结构相比，减少了匹配多个链化学计量的负担，并且得到了结构灵活的瓦片。作为一个应用实例，我们已经表明，四螺旋编织瓦片可用于体外提高凝血酶结合适体的抗凝血活性。
