Department of Computer Science, Duke University, Durham, North Carolina 27708, United States.
J Am Chem Soc. 2011 Mar 23;133(11):3843-5. doi: 10.1021/ja1108886. Epub 2011 Feb 28.
DNA is a useful material for nanoscale construction. Due to highly specific Watson-Crick base pairing, the DNA sequences can be designed to form small tiles or origami. Adjacent helices in such nanostructures are connected via Holliday junction-like crossovers. DNA tiles can have sticky ends which can then be programmed to form large one-dimensional and two-dimensional periodic lattices. Recently, a three-dimensional DNA lattice has also been constructed. Here we report the design and construction of a novel DNA cross tile, called the double-decker tile. Its arms are symmetric and have four double helices each. Using its sticky ends, large two-dimensional square lattices have been constructed which are on the order of tens of micrometers. Furthermore, it is proposed that the sticky ends of the double-decker tile can be programmed to form a three-dimensional periodic lattice with large cavities that could be used as a scaffold for precise positioning of molecules in space.
DNA 是一种用于纳米级构建的有用材料。由于高度特异性的 Watson-Crick 碱基配对，DNA 序列可以被设计成形成小瓦片或折纸。这种纳米结构中的相邻螺旋通过类似于 Holliday 连接的交叉连接。DNA 瓦片可以有粘性末端，然后可以编程形成大的一维和二维周期性晶格。最近，也构建了一个三维 DNA 晶格。在这里，我们报告了一种新型 DNA 十字瓦片的设计和构建，称为双层瓦片。它的臂是对称的，每个臂有四个双螺旋。使用其粘性末端，已构建了大型二维正方形晶格，其尺寸约为数十微米。此外，有人提出，双层瓦片的粘性末端可以被编程形成具有大空腔的三维周期性晶格，该晶格可作为分子在空间中精确定位的支架。
