Department of Stomatology, the Affiliated Suzhou Municipal Hospital of Nanjing Medical University, Suzhou, China.
Acta Odontol Scand. 2011 Jan;69(1):27-32. doi: 10.3109/00016357.2010.517555. Epub 2010 Sep 23.
To investigate whether short-term daily consumption of yoghurt leads to colonization by Lactobacillus acidophilus in a group of human subjects who were initially totally devoid of L. acidophilus in their oral cavities.
Twenty-three volunteers consumed yogurt containing L. acidophilus during a 14-day trial stage. Oral and fecal samples were collected at the clearance stage and at the post-yoghurt intake stage until L. acidophilus was found. Standard polymerase chain reaction methods using specific primers were adopted for the detection and identification of L. acidophilus.
The isolation frequency decreased rapidly 72 h after stopping intake of yoghurt. After 1 week, L. acidophilus was absent in all oral samples. Non-significant differences were found between the survival rates of L. acidophilus in samples of saliva, plaque, tongue surface, and buccal mucosa. L. acidophilus was also found to remain in the gastrointestinal tract for longer than in the oral cavity.
Allochthonous L. acidophilus is not likely to permanently colonize the oral cavity and intestine.
研究短期每日食用酸奶是否会导致一组口腔内最初完全没有嗜酸乳杆菌的人体受试者发生嗜酸乳杆菌定植。
23 名志愿者在为期 14 天的试验阶段食用含有嗜酸乳杆菌的酸奶。在清除阶段和酸奶摄入后阶段采集口腔和粪便样本，直到发现嗜酸乳杆菌。采用特异性引物的标准聚合酶链反应方法用于检测和鉴定嗜酸乳杆菌。
停止摄入酸奶 72 小时后，分离频率迅速下降。1 周后，所有口腔样本中均未发现嗜酸乳杆菌。唾液、牙菌斑、舌面和颊黏膜样本中嗜酸乳杆菌的存活率无显著差异。还发现嗜酸乳杆菌在胃肠道中的存活时间长于口腔。
异源嗜酸乳杆菌不太可能永久定植于口腔和肠道。
