Department of Civil and Environmental Engineering, The University of Tennessee, Knoxville, TN 37996, USA.
Bioresour Technol. 2011 Jan;102(2):779-85. doi: 10.1016/j.biortech.2010.08.104. Epub 2010 Sep 21.
The objective of this study is to investigate the responses of methanogen populations to poultry waste addition by comparing the archaeal microbial populations in continuous anaerobic digesters with or without the addition of poultry waste as a co-substrate. Poultry waste was characterized as an organic/nitrogen-rich substrate for anaerobic digestion. Supplementing dilute dairy waste with poultry waste for anaerobic co-digestion to increase organic loading rate by 50% resulted in improved biogas production. Elevated ammonia derived from poultry waste did not lead to process inhibition at the organic loadings tested, demonstrating the feasibility of the anaerobic co-digestion of dairy and poultry wastes for improved treatment efficiency. The stability of the anaerobic co-digestion process was linked to the robust archaeal microbial community, which remained mostly unchanged in community structure following increases in organic loading and ammonia levels. Surprisingly, Crenarchaeota archaeal populations, instead of the Euryarchaeota methanogens, dominated the archaeal communities in the anaerobic digesters. The ecological functions of these abundant non-methanogen archaeal populations in anaerobic digestion remain to be identified.
本研究旨在通过比较添加和不添加禽畜废物作为共基质的连续厌氧消化器中古菌微生物种群，来探究产甲烷菌种群对禽畜废物添加的响应。禽畜废物被认为是一种适合于厌氧消化的富含有机物/氮的基质。将禽畜废物添加到稀释的乳制品废物中，使有机负荷率提高 50%，从而提高沼气产量。在测试的有机负荷条件下，禽畜废物产生的氨并没有导致处理过程受到抑制，这表明禽畜废物和乳制品废物的厌氧共消化具有提高处理效率的可行性。厌氧共消化过程的稳定性与微生物群落结构的稳定性有关，在有机负荷和氨水平增加后，微生物群落结构基本保持不变。令人惊讶的是，在厌氧消化器中，Crenarchaeota 古菌种群而不是 Euryarchaeota 产甲烷菌占据了古菌群落的主导地位。这些丰富的非产甲烷古菌种群在厌氧消化中的生态功能仍有待确定。
