Chen Si, He Qiang
Department of Civil and Environmental Engineering, The University of Tennessee, Knoxville, TN, USA.
J Ind Microbiol Biotechnol. 2015 Aug;42(8):1129-37. doi: 10.1007/s10295-015-1632-7. Epub 2015 May 9.
Anaerobic digestion is a sustainable technology for the treatment of organic waste and production of biogas. Acetoclastic methanogenesis accounts for the majority of methane production in anaerobic digestion. Therefore, sustaining robust acetoclastic methanogens is important for stable process performance. Due to faster growth kinetics at high acetate concentrations, it has been considered that Methanosarcina would be more prevalent than Methanosaeta in unstable anaerobic digestion processes which frequently experience high acetate levels. Methanogen population dynamics were monitored in multiple continuous anaerobic digesters for 500 days. Results from quantitative polymerase chain reaction analysis show that Methanosaeta dominated over Methanosarcina in anaerobic digestion at high acetate levels up to 44 mM, suggesting the potential of Methanosaeta as a robust and efficient acetoclastic candidate for resilient anaerobic methane conversion. Further efforts are needed to identify mechanisms contributing to the unexpected competitiveness of these methanogens at high acetate levels observed in this study.
厌氧消化是一种用于处理有机废物和生产沼气的可持续技术。乙酸裂解产甲烷作用在厌氧消化产生的甲烷中占大部分。因此，维持活跃的乙酸裂解产甲烷菌对于稳定的工艺性能很重要。由于在高乙酸盐浓度下具有更快的生长动力学，人们认为在经常经历高乙酸盐水平的不稳定厌氧消化过程中，甲烷八叠球菌比甲烷丝状菌更普遍。在多个连续厌氧消化器中监测产甲烷菌种群动态达500天。定量聚合酶链反应分析结果表明，在高达44 mM的高乙酸盐水平下的厌氧消化中，甲烷丝状菌比甲烷八叠球菌占优势，这表明甲烷丝状菌作为一种强大且高效的乙酸裂解候选菌，具有实现弹性厌氧甲烷转化的潜力。需要进一步努力来确定导致本研究中观察到的这些产甲烷菌在高乙酸盐水平下具有意外竞争力的机制。
