Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar-608002, Tamil Nadu, India.
Eur J Pharmacol. 2011 Jan 10;650(1):465-71. doi: 10.1016/j.ejphar.2010.08.059. Epub 2010 Sep 19.
The present study aims to evaluate the antihyperglycaemic, antilipid peroxidative and antioxidant effects of gallic acid on streptozotocin induced diabetic male Wistar rats. To induce diabetes mellitus, rats were injected with streptozotocin intraperitoneally at a single dose of 40mg/kg. Streptozotocin induced diabetic rats showed significant (P<0.05) increase in the levels of blood glucose, glycosylated haemoglobin and significant (P<0.05) decrease in the levels of plasma insulin, body weight and total haemoglobin. Diabetic rats also showed significant (P<0.05) decrease in the activity of hepatic hexokinase and significant (P<0.05) increase in the activities of glucose-6-phosphatase and fructose-1, 6-bisphosphatase. The pancreatic thiobarbituric acid reactive substances and lipid hydroperoxides were significantly (P<0.05) increased and the activities of pancreatic superoxide dismutase, catalase and glutathione peroxidase were significantly (P<0.05) decreased in diabetic rats. Oral treatment with gallic acid (10 and 20mg/kg) daily for a period of 21days showed significant (P<0.05) protective effects on all the biochemical parameters studied. Histopathology of pancreas confirmed the protective effects of gallic acid in diabetic rats. In vitro study also revealed the potent antioxidant effect of gallic acid. Thus, the study shows the antihyperglycaemic, antilipid peroxidative and antioxidant effects of gallic acid on streptozotocin induced diabetic rats. The effect exerted by 20mg/kg body weight of gallic acid was more effective than 10mg/kg body weight of gallic acid.
本研究旨在评估没食子酸对链脲佐菌素诱导的雄性 Wistar 糖尿病大鼠的降血糖、抗脂质过氧化和抗氧化作用。为了诱导糖尿病，大鼠一次性腹腔注射链脲佐菌素 40mg/kg。链脲佐菌素诱导的糖尿病大鼠血糖、糖化血红蛋白水平显著升高（P<0.05），血浆胰岛素、体重和总血红蛋白水平显著降低（P<0.05）。糖尿病大鼠肝己糖激酶活性显著降低（P<0.05），葡萄糖-6-磷酸酶和果糖-1,6-二磷酸酶活性显著升高（P<0.05）。糖尿病大鼠胰腺硫代巴比妥酸反应物和脂质过氧化物明显增加（P<0.05），胰腺超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性明显降低（P<0.05）。每日口服没食子酸（10 和 20mg/kg）21 天，对所有研究的生化参数均有显著的保护作用（P<0.05）。胰腺组织病理学证实了没食子酸对糖尿病大鼠的保护作用。体外研究也揭示了没食子酸的强大抗氧化作用。因此，本研究表明没食子酸对链脲佐菌素诱导的糖尿病大鼠具有降血糖、抗脂质过氧化和抗氧化作用。20mg/kg 体重没食子酸的作用比 10mg/kg 体重没食子酸更有效。
