Pfizer, Tissue Repair, 200 CambridgePark Drive, Cambridge, MA 02140, USA.
Osteoarthritis Cartilage. 2010 Oct;18 Suppl 3:S17-23. doi: 10.1016/j.joca.2010.05.025.
To describe a histologic scoring system for murine osteoarthritis (OA) that can be applied universally to instability, enzymatic, transgenic and spontaneous OA models.
Scientists with expertise in assessing murine OA histopathology reviewed the merits and drawbacks of methods described in the literature. A semi-quantitative scoring system that could reasonably be employed in any basic cartilage histology laboratory was proposed. This scoring system was applied to a set of 10 images of the medial tibial plateau and femoral condyle to yield 20 scores. These images were scored twice by four experienced scorers (CL, SG, MC, TA), with a minimum time interval of 1 week between scores to obtain intra-observer variability. An additional three novice scorers (CR, CL and MM) with no previous experience evaluated the images to determine the ease of use and reproducibility across laboratories.
The semi-quantitative scoring system was relatively easy to apply for both experienced and novice scorers and the results had low inter- and intra-scorer variability. The variation in scores across both the experienced and novice scorers was low for both tibia and femur, with the tibia always having greater consistency.
The semi-quantitative scoring system recommended here is simple to apply and required no specialized equipment. Scoring of the tibial plateaus was highly reproducible and more consistent than that of the femur due to the much thinner femoral cartilage. This scoring system may be a useful tool for both new and experienced scorers to sensitively evaluate models and OA mechanisms, and also provide a common paradigm for comparative evaluation across the many groups performing these analyses.
描述一种可普遍应用于不稳定、酶、转基因和自发性骨关节炎模型的鼠类骨关节炎（OA）组织学评分系统。
具有评估鼠类 OA 组织病理学专业知识的科学家审查了文献中描述的方法的优缺点。提出了一种半定量评分系统，该系统可以在任何基本的软骨组织学实验室中合理使用。该评分系统应用于一组 10 张内侧胫骨平台和股骨髁的图像，得出 20 个评分。这组图像由 4 名有经验的评分者（CL、SG、MC、TA）进行了两次评分，两次评分的间隔至少为 1 周，以获得观察者内变异性。另外 3 名没有先前经验的新手评分者（CR、CL 和 MM）评估了这些图像，以确定在不同实验室之间的易用性和可重复性。
半定量评分系统对于有经验和新手评分者来说都相对容易应用，并且结果具有较低的观察者内和观察者间变异性。对于胫骨和股骨，经验丰富和新手评分者的评分变化都较低，胫骨的一致性始终较高。
这里推荐的半定量评分系统易于应用，且不需要专门的设备。胫骨平台的评分具有高度的可重复性，且比股骨更为一致，这是由于股骨软骨更薄。该评分系统可能是新老评分者敏感评估模型和 OA 机制的有用工具，并且还为进行这些分析的许多组提供了跨组比较评估的通用范例。
