Lee Hyun Sook, Jung Jae In, Hur NaYon, Bae Jaewoo, Lee Jae Kyoung, Kim Eun Ji
Department of Food Science & Nutrition, Dongseo University, Busan 47011, Korea.
Industry Coupled Cooperation Center for Bio Healthcare Materials, Hallym University, Chuncheon 24252, Korea.
Nutr Res Pract. 2025 Aug;19(4):537-553. doi: 10.4162/nrp.2025.19.4.537. Epub 2025 Mar 10.
BACKGROUNDS/OBJECTIVES: This study examined the anti-osteoarthritic effects of a Linn ethanol extract (CLE), standardized to 500 mg/g of curcuminoids, in monosodium iodoacetate (MIA)-induced osteoarthritis (OA) Sprague-Dawley (SD) rats.
MATERIALS/METHODS: The rats were divided into a normal control group (NC), OA control group (OC), OA +10, 20, 30, or 50 mg/kg body weight (BW)/day CLE-administered group (O+CL10, O+CL20, O+CL30, or O+CL50), and OA+150 mg/kg BW/day methyl sulfonyl methane (MSM, positive control)-administered group (O+M). The rats were given oral doses of CLE or MSM for 5 weeks, with an intra-articular injection of MIA into their knee joints on the 15th day to induce OA. Micro-computed tomography, histological examination, and immunofluorescence staining were performed on the knee joint. The inflammatory mediators, cytokines, and matrix metalloproteinase (MMP) mRNA expression were measured in the knee joint synovial fluid.
The OC group showed more severe knee joint swelling and subchondral bone erosion than the NC group, and aggrecan and type II collagen expression in the articular cartilage was reduced. The OC group exhibited lower mRNA expression of inflammatory mediators, cytokines, and MMPs than the NC group. In OA-induced rats, the oral administration of the CLE reduced knee swelling and cartilage degradation and increased aggrecan and type II collagen expression in the articular cartilage. CLE administration reduced the expression of inducible nitric oxide synthase, cyclooxygenase-2, 5-lipoxygenase, interleukin (IL)-1β, IL-6, tumor necrosis factor-α, MMP-2, MMP-3, MMP-9, and MMP-13 in the synovia of knee joints. In particular, these levels reached the same as the positive control group, the O+M group.
The CLE had potent anti-OA effects in MIA-induced OA rats by suppressing the expression of inflammatory mediators, cytokines, and MMPs, alleviating cartilage degradation. Hence, the CLE is a potential functional food ingredient that can prevent and improve OA.
背景/目的：本研究考察了姜黄乙醇提取物（CLE，姜黄素类化合物含量为500毫克/克）对碘乙酸钠（MIA）诱导的骨关节炎（OA）的Sprague-Dawley（SD）大鼠的抗骨关节炎作用。
材料/方法：将大鼠分为正常对照组（NC）、OA对照组（OC）、OA + 10、20、30或50毫克/千克体重（BW）/天CLE给药组（O + CL10、O + CL20、O + CL30或O + CL50）以及OA + 150毫克/千克BW/天甲磺酰甲烷（MSM，阳性对照）给药组（O + M）。大鼠口服CLE或MSM 5周，于第15天膝关节腔内注射MIA诱导OA。对膝关节进行微计算机断层扫描、组织学检查和免疫荧光染色。检测膝关节滑液中炎症介质、细胞因子和基质金属蛋白酶（MMP）的mRNA表达。
与NC组相比，OC组膝关节肿胀和软骨下骨侵蚀更严重，关节软骨中聚集蛋白聚糖和II型胶原表达降低。与NC组相比，OC组炎症介质、细胞因子和MMPs的mRNA表达较低。在OA诱导的大鼠中，口服CLE可减轻膝关节肿胀和软骨降解，并增加关节软骨中聚集蛋白聚糖和II型胶原表达。给予CLE可降低膝关节滑膜中诱导型一氧化氮合酶、环氧化酶-2、5-脂氧合酶、白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α、MMP-2、MMP-3、MMP-9和MMP-13的表达。特别是，这些水平与阳性对照组O + M组相同。
CLE通过抑制炎症介质、细胞因子和MMPs的表达，减轻软骨降解，对MIA诱导的OA大鼠具有有效的抗OA作用。因此，CLE是一种潜在的可预防和改善OA的功能性食品成分。
