Mihara M, Ohsugi Y
Fuji-Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd., Shizuoka, Japan.
Int Arch Allergy Appl Immunol. 1990;93(1):89-92. doi: 10.1159/000235285.
This study demonstrates that interleukin-6 (IL-6) increases the autoantibody production by B cells from NZB/W F1 mice. Splenic B cells were cultured for 5 days in the presence or absence of human IL-6, and then the anti-DNA antibody and immunoglobulin contents in the culture supernatants were measured by ELISA. Adding IL-6 increased IgG anti-DNA antibody production by B cells from old mice (30 weeks), but not from young ones (17 weeks). B cells obtained from both young and old mice produced IgM anti-DNA antibody, which increased when IL-6 was added. The increased anti-DNA antibody production was suppressed by anti-recombinant human IL-6 antibody to the background level, i.e. antibody contents in the absence of IL-6. In contrast, murine IL-5 did not increase IgG anti-DNA antibody production, although it promoted the production of IgM anti-DNA antibody. Furthermore, when IL-5 was added in combination with IL-6, there was an additive increase in IgM, but not in IgG anti-DNA antibody production. Similar results were obtained in the measurement of the immunoglobulin contents. These results suggest the possible role of IL-6 in the pathogenesis of autoimmune disease in NZB/W F1 mice.
本研究表明，白细胞介素-6（IL-6）可增加NZB/W F1小鼠B细胞的自身抗体产生。将脾B细胞在有或无人类IL-6的情况下培养5天，然后通过酶联免疫吸附测定法（ELISA）测量培养上清液中的抗DNA抗体和免疫球蛋白含量。添加IL-6可增加老年小鼠（30周龄）B细胞产生IgG抗DNA抗体，但对年轻小鼠（17周龄）的B细胞无此作用。从年轻和老年小鼠获得的B细胞均产生IgM抗DNA抗体，添加IL-6后其产量增加。抗重组人IL-6抗体可将增加的抗DNA抗体产生抑制至背景水平，即在无IL-6时的抗体含量水平。相比之下，鼠IL-5虽能促进IgM抗DNA抗体的产生，但不会增加IgG抗DNA抗体的产生。此外，当IL-5与IL-6联合添加时，IgM抗DNA抗体产量呈累加增加，但IgG抗DNA抗体产量无此变化。在免疫球蛋白含量测量中也得到了类似结果。这些结果提示IL-6在NZB/W F1小鼠自身免疫性疾病发病机制中可能发挥的作用。
