Molecular Discovery Research, GlaxoSmithKline, 1250 S Collegeville Road, Collegeville, PA 19426, USA.
Mol Biotechnol. 2011 Mar;47(3):270-85. doi: 10.1007/s12033-010-9335-9.
High throughput screening (HTS) is at the core of the drug discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful analysis of many variables, starting with the choice of assay target and ending with the discovery of lead compounds. At every step in this process, there are decisions to be made that can greatly impact the outcome of the HTS effort, to the point of making it a success or a failure. Although specific guidelines should be established to insure that the screening assay reaches an acceptable level of quality, many choices require pragmatism and the ability to compromise opposing forces.
高通量筛选（HTS）是药物发现过程的核心，因此，设计和实施全面的 HTS 测定法至关重要，需要生物学、化学、工程和信息学等领域的科学家共同参与。这需要仔细分析许多变量，从测定法目标的选择开始，一直到先导化合物的发现。在这个过程的每一步，都需要做出决策，这些决策可能会极大地影响 HTS 工作的结果，甚至决定成败。虽然应该制定具体的指南来确保筛选测定法达到可接受的质量水平，但许多选择需要实用主义和妥协对立力量的能力。
