Shaposhnikov Ia D, Ratovitskiĭ E A, Kuznetsov O K
Biull Eksp Biol Med. 1978 Mar;85(3):332-4.
The subcellular localization in chicken Rous sarcoma of nucleotide sequence, complementary to Rous sarcoma virus RNA was examined by RNA/RNA molecular hybridization. The preparations of radioiodinated virion RNA were annealed with RNAs from different fractions (nuclei, mitochondria, free and membrane-bound polyribosomes) isolated from chicken Rous sarcoma. Formation of RNA-ase resistant hybrids between the viral 125I-RNA and RNA from the mitochondria and membrane-bound polyribosomes was revealed. The latter were characterized by a higher relative redundancy of nucleotide sequences complementary to virion RNA than that in the former, by factor 446. The role of complementary ribonucleotide sequences is discussed.
通过RNA/RNA分子杂交技术，对鸡劳斯肉瘤中与劳斯肉瘤病毒RNA互补的核苷酸序列进行了亚细胞定位研究。用放射性碘标记的病毒粒子RNA制剂与从鸡劳斯肉瘤中分离出的不同组分（细胞核、线粒体、游离和膜结合多核糖体）的RNA进行退火处理。结果显示，病毒的125I-RNA与线粒体和膜结合多核糖体的RNA之间形成了RNA酶抗性杂交体。后者与病毒粒子RNA互补的核苷酸序列相对冗余度比前者高446倍。文中还讨论了互补核糖核苷酸序列的作用。
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