Biorefining Research Initiative, Lakehead University, Thunder Bay, Ontario, Canada.
Crit Rev Biotechnol. 2010 Dec;30(4):302-9. doi: 10.3109/07388551.2010.490938. Epub 2010 Sep 24.
Cellulose, the major constituent of all plant materials and the most abundant organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected by β-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases), and β-glucosidases acting in a synergistic manner. In biopolymer hydrolysis studies, enzyme assay is an indispensable part. The most commonly used assays for the individual enzymes as well as total cellulase activity measurements, including their advantages and limitations, are summarized in this review article. In addition, some novel approaches recently used for enzyme assays are summarized.
纤维素是所有植物材料的主要成分，也是地球上最丰富的有机分子，它是由葡萄糖分子通过β-1,4-糖苷键连接而成的线性生物聚合物。纤维素的酶解需要包括内切葡聚糖酶、外切葡聚糖酶（纤维二糖水解酶）和β-葡萄糖苷酶在内的水解酶混合物，以协同方式发挥作用。在生物聚合物水解研究中，酶测定是不可或缺的一部分。本文综述了用于测定各种酶以及总纤维素酶活性的最常用的测定方法，包括它们的优缺点。此外，还总结了最近用于酶测定的一些新方法。
