Department of Anatomy, University of California at San Francisco, 1550 4th Street, San Francisco, CA 94158, USA.
Curr Opin Struct Biol. 2010 Oct;20(5):623-31. doi: 10.1016/j.sbi.2010.08.008. Epub 2010 Sep 24.
X-ray imaging of biological samples is progressing rapidly. In this paper we review the progress to date in high-resolution imaging of cellular architecture. In particular we survey the progress in soft X-ray tomography and argue that the field is coming of age and that important biological insights are starting to emerge. We then review the new ideas based on coherent diffraction. These methods are at a much earlier stage of development but, as they eliminate the need for X-ray optics, have the capacity to provide substantially better spatial resolution than zone plate-based methods.
生物样本的 X 射线成像是在迅速发展的。在本文中，我们回顾了目前在细胞结构的高分辨率成像方面的进展。特别地，我们调查了软 X 射线断层摄影术的进展，并认为该领域正在走向成熟，并且开始出现重要的生物学见解。然后，我们回顾了基于相干衍射的新思想。这些方法还处于发展的早期阶段，但是由于它们不需要 X 射线光学器件，因此具有比基于波带板的方法提供更高的空间分辨率的潜力。
