Adamczyk-Szabela Dorota, Anielak Piotr, Wolf Wojciech M
Institute of General and Ecological Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.
J Anal Methods Chem. 2017;2017:6947376. doi: 10.1155/2017/6947376. Epub 2017 Oct 19.
Mineralization to the complete oxidation of sample carbon component does not always assure the best analyte recovery. Particular attention should be paid to the presence of silicon in the investigated plant sample and especially in the certified reference material for which Si content is scarcely given by the providers. During mineralization without addition of the hydrofluoric acid, the residual carbon may block silica surfaces and increase availability of an analyte for its spectral determination in the solution. This issue is of particular relevance because standard protocols for digestion of plant matrices often do not support hydrofluoric acid addition. Several procedures recommended for decomposition of herbal plants were applied for the respective certified reference material and examined in detail. Manganese, copper, and zinc contents were analyzed in all samples by the flame atomic absorption spectrometry. Additionally, the residual carbon was determined in all mineralizates. Silicon content was analyzed by the X-ray fluorescence method. The best recoveries were observed for samples characterized by relatively high residual carbon.
将样品碳成分完全氧化成矿化并不总能确保最佳的分析物回收率。应特别注意被调查植物样品中硅的存在，尤其是在认证参考物质中，供应商很少给出其硅含量。在不添加氢氟酸的矿化过程中，残留碳可能会堵塞二氧化硅表面，并增加分析物在溶液中进行光谱测定的可利用性。这个问题特别重要，因为植物基质消解的标准方案通常不支持添加氢氟酸。将推荐用于分解草药植物的几种程序应用于各自的认证参考物质并进行了详细检查。通过火焰原子吸收光谱法分析了所有样品中的锰、铜和锌含量。此外，还测定了所有矿化产物中的残留碳。通过X射线荧光法分析硅含量。对于具有相对较高残留碳的样品，观察到了最佳回收率。
