Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova 35100 Izmir, Turkey.
Necmettin Erbakan University, Faculty of A. K. Education, Science and Technology Department, 42090 Konya, Turkey.
Biosens Bioelectron. 2017 Jun 15;92:207-214. doi: 10.1016/j.bios.2017.02.016. Epub 2017 Feb 11.
Recently, the low-cost effective biosensing systems based on advanced nanomaterials have received a key attention for development of novel assays for rapid and sequence-specific nucleic acid detection. The electrochemical biosensor based on reduced graphene oxide (rGO) modified disposable pencil graphite electrodes (PGEs) were developed herein for electrochemical monitoring of DNA, and also for monitoring of biointeraction occurred between anticancer drug, Daunorubicin (DNR), and DNA. First, rGO was synthesized chemically and characterized by using UV-Vis, TGA, FT-IR, Raman Spectroscopy and SEM techniques. Then, the quantity of rGO assembling onto the surface of PGE by passive adsorption was optimized. The electrochemical behavior of rGO-PGEs was examined by cyclic voltammetry (CV). rGO-PGEs were then utilized for electrochemical monitoring of surface-confined interaction between DNR and DNA using differential pulse voltammetry (DPV) technique. Additionally, voltammetric results were complemented with electrochemical impedance spectroscopy (EIS) technique. Electrochemical monitoring of DNR and DNA was resulted with satisfying detection limits 0.55µM and 2.71µg/mL, respectively.
最近，基于先进纳米材料的低成本有效生物传感系统受到了关注，用于开发新型分析方法，实现快速和序列特异性核酸检测。本文构建了基于还原氧化石墨烯(rGO)修饰的一次性铅笔石墨电极(PGE)的电化学生物传感器，用于电化学监测 DNA，以及监测抗癌药物柔红霉素(DNR)与 DNA 之间发生的生物相互作用。首先，通过使用紫外-可见分光光度法、热重分析(TGA)、傅里叶变换红外光谱(FT-IR)、拉曼光谱和扫描电子显微镜(SEM)技术，对 rGO 进行了化学合成和表征。然后，优化了 rGO 通过被动吸附组装到 PGE 表面的量。通过循环伏安法(CV)对 rGO-PGE 的电化学行为进行了研究。然后，利用差分脉冲伏安法(DPV)技术，rGO-PGE 用于电化学监测 DNR 和 DNA 表面受限相互作用。此外，通过电化学阻抗谱(EIS)技术补充了伏安法结果。DNR 和 DNA 的电化学监测分别得到了令人满意的检测限 0.55µM 和 2.71µg/mL。
