Laboratory of Photonic Chinese Medicine, College of Biophotonics, South China Normal University, Guangzhou 510631, People's Republic of China.
Phys Med Biol. 2010 Oct 21;55(20):6175-85. doi: 10.1088/0031-9155/55/20/009. Epub 2010 Sep 29.
Ultraviolet B (UVB) has been widely used in dermatological phototherapy. Narrowband UVB (NB-UVB), with a peak at 311 nm, is considered to be more effective than broadband UVB (BB-UVB). However, the safety of NB-UVB is controversial. In this study, we first introduced optical coherence tomography (OCT), a novel, non-invasive in vivo imaging technology, to assess the effect of NB-UVB and BB-UVB on skin. Balb/c mice dorsal skin was exposed with increasing UVB doses (1MED, 3MEDs and 5MEDs), and then OCT images of the tissues were obtained by an OCT system with 1310 nm central wavelength. Quantitative parameters (skin thickness, disruption of the entrance signal and correlation coefficient) were extracted from the OCT images. The data indicated that NB-UVB-induced skin lesions were similar to that of BB-UVB at 1MED or 3MEDs UVB. However, the skin tissues exposed with 5MEDs NB-UVB suffered from more lesions than BB-UVB. Furthermore, the persistence of skin inflammation in 3MEDs NB-UVB-induced skin tissues was much longer than that of BB-UVB (P = 0.004). In conclusion, optimized treatment time and frequency as well as close clinical monitoring should be undertaken to reduce the latent risk of NB-UVB phototherapy.
紫外线 B（UVB）已广泛应用于皮肤科光疗。窄谱 UVB（NB-UVB），峰值在 311nm，被认为比广谱 UVB（BB-UVB）更有效。然而，NB-UVB 的安全性存在争议。在这项研究中，我们首先引入了光学相干断层扫描（OCT），这是一种新型的、非侵入性的体内成像技术，来评估 NB-UVB 和 BB-UVB 对皮肤的影响。Balb/c 小鼠背部皮肤接受递增的 UVB 剂量（1MED、3MEDs 和 5MEDs）照射，然后使用中心波长为 1310nm 的 OCT 系统获得组织的 OCT 图像。从 OCT 图像中提取定量参数（皮肤厚度、入射信号的中断和相关系数）。数据表明，NB-UVB 诱导的皮肤损伤在 1MED 或 3MEDs UVB 时与 BB-UVB 相似。然而，接受 5MEDs NB-UVB 照射的皮肤组织的损伤比 BB-UVB 更严重。此外，3MEDs NB-UVB 诱导的皮肤组织中皮肤炎症的持续时间比 BB-UVB 长得多（P = 0.004）。总之，应该采取优化的治疗时间和频率以及密切的临床监测，以降低 NB-UVB 光疗的潜在风险。
