Walz Anna-Lena, Liebl Lana, Schmitt Katrin, Scharfer Philip, Schabel Wilhelm
Institute of Thermal Process Engineering Thin Film Technology Karlsruhe Institute of Technology Karlsruhe Germany.
Eng Life Sci. 2019 Aug 29;19(11):741-748. doi: 10.1002/elsc.201900114. eCollection 2019 Nov.
In the light of continuous improvement and optimization, recent experiments that the authors conducted give new insights into the applied evaluation method of Riegel et al. [1]: Thorough investigations of the previous results regarding the usage of the Lowry Assay showed discrepancies in the determination of the released amount of protein in the analysis solution. The accurate quantification of this parameter is crucial as it directly influences the calculation of the residual enzymatic activity. In concrete terms, this finding has a major impact on the presented and discussed results in the article "Activity determination of FAD-dependent glucose dehydrogenase immobilized in PEDOT: PSS-PVA composite films for biosensor applications" [1]. Thus, this commentary addresses the new insights concerning the applied evaluation method, explains necessary revisions and discusses new conclusions derived from the adjusted evaluation method.
鉴于持续改进和优化，作者最近进行的实验为Riegel等人[1]应用的评估方法提供了新的见解：对先前关于Lowry测定法使用结果的深入研究表明，在分析溶液中蛋白质释放量的测定上存在差异。该参数的准确定量至关重要，因为它直接影响残余酶活性的计算。具体而言，这一发现对文章《用于生物传感器应用的固定在PEDOT:PSS-PVA复合膜中的FAD依赖性葡萄糖脱氢酶的活性测定》[1]中呈现和讨论的结果有重大影响。因此，本评论阐述了关于应用评估方法的新见解，解释了必要的修订，并讨论了从调整后的评估方法得出的新结论。
