Lorena Nádia Suely de Oliveira, Pitombo Marcos Bettini, Côrtes Patrícia Barbur, Maya Maria Cristina Araújo, Silva Marlei Gomes da, Carvalho Ana Carolina da Silva, Coelho Fábrice Santana, Miyazaki Neide Hiromi Tokumaru, Marques Elizabeth Andrade, Chebabo Alberto, Freitas Andréa D'Avila, Lupi Otília, Duarte Rafael Silva
Medical Sciences Faculty, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Acta Cir Bras. 2010 Oct;25(5):455-9. doi: 10.1590/s0102-86502010000500013.
To evaluate the minimum inhibitory concentration (MIC) of GTA against these microorganisms and alternative disinfectants for high-level disinfection (HLD).
Reference mycobacteria and clinical M. massiliense strains were included in this study. Active cultures were submitted to susceptibility qualitative tests with GTA dilutions (ranging from 1.5% to 8%), and commercial orthophthaldehyde (OPA) and peracetic acid (PA)-based solutions, during the period of exposure as recommended by National Agency of Sanitary Surveillance for HLD.
All reference and M. massiliense non-BRA100 strains, recovered from sputum, were susceptible to any GTA concentration, OPA and PA solutions. M. massiliense BRA100 strains presented MIC of 8% GTA and were susceptible to OPA and PA.
M. massiliense BRA100 strain is resistant to high GTA concentrations (up to 7%), which proves that this product is non-effective against specific rapidly growing mycobacteria and should be substituted by OPA or PA-based solutions for HLD.
评估胍类消毒剂（GTA）对这些微生物的最低抑菌浓度（MIC）以及用于高水平消毒（HLD）的替代消毒剂。
本研究纳入了参考分枝杆菌和马赛分枝杆菌临床菌株。将活性培养物按照国家卫生监督机构推荐的高水平消毒暴露时间，用GTA稀释液（浓度范围为1.5%至8%）、市售邻苯二甲醛（OPA）和过氧乙酸（PA）溶液进行药敏定性试验。
所有从痰液中分离出的参考菌株和非BRA100型马赛分枝杆菌菌株，对任何GTA浓度、OPA和PA溶液均敏感。BRA100型马赛分枝杆菌菌株对GTA的MIC为8%，对OPA和PA敏感。
BRA100型马赛分枝杆菌菌株对高浓度GTA（高达7%）耐药，这证明该产品对特定快速生长的分枝杆菌无效，高水平消毒应使用OPA或PA溶液替代。
