Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Brazil.
Braz Oral Res. 2010 Jul-Sep;24(3):342-8. doi: 10.1590/s1806-83242010000300014.
This study evaluated the long-term efficacy of denture cleansers against Candida spp. biofilm recolonization on liner surface. Specimens were fabricated of a poly(methyl methacrylate)-based denture liner and had their surface roughness evaluated at baseline and after cleansing treatments. C. albicans or C. glabrata biofilms were formed on liner surface for 48 h, and then the specimens were randomly assigned to one of cleaning treatments: two alkaline peroxides (soaking for 3 or 15 min), 0.5% sodium hypochlorite (10 min) or distilled water (control; 15 min). After the treatments, the specimens were sonicated to disrupt the biofilm, and residual cells were counted (cell/mL). Long-term effectiveness of the cleaning processes was determined by submitting a set of cleaned specimens to biofilm growth conditions for 48 h followed by estimation of cell counts. The topography of specimens after cleaning treatments was analyzed by SEM. Data were analyzed by ANOVA and Tukey's test (α; = 0.05). Results of cell count estimation showed significant differences in cleanliness among the treatments (p < 0.001), and it could be observed by SEM. However, no significant difference (p > 0.05) was observed among the Candida species regarding the recolonization condition. Alkaline denture cleansers showed similar cleaning performance and both differed from the control (p < 0.001). Sodium hypochlorite was the only treatment that removed biofilm efficiently, since no viable cells were found after its use. In conclusion, alkaline peroxide denture cleansers were not effective in removing Candida spp. biofilm from denture liner surfaces and preventing biofilm recolonization.
本研究评估了假牙清洁剂对白色念珠菌和光滑念珠菌生物膜在义齿衬里表面再定植的长期疗效。标本由聚甲基丙烯酸甲酯基义齿衬里制成，在基线和清洁处理后评估其表面粗糙度。在衬里表面形成白色念珠菌或光滑念珠菌生物膜 48 小时，然后将标本随机分配到一种清洁处理中：两种碱性过氧化物（浸泡 3 或 15 分钟）、0.5%次氯酸钠（10 分钟）或蒸馏水（对照；15 分钟）。处理后，用超声波处理标本以破坏生物膜，并计算残留细胞数（细胞/ml）。通过将一组清洁后的标本提交给生物膜生长条件 48 小时来确定清洁过程的长期效果，然后估计细胞计数。用 SEM 分析清洁处理后标本的形貌。数据采用方差分析和 Tukey 检验（α；=0.05）进行分析。细胞计数估计结果显示，处理之间的清洁度有显著差异（p<0.001），并用 SEM 观察到。然而，对于再定植条件，不同念珠菌种之间没有观察到显著差异（p>0.05）。碱性假牙清洁剂具有相似的清洁性能，与对照组相比均有差异（p<0.001）。次氯酸钠是唯一能够有效去除生物膜的处理方法，因为使用后未发现存活细胞。总之，碱性过氧化物假牙清洁剂不能有效去除义齿衬里表面的白色念珠菌生物膜并防止生物膜再定植。
