Department of Prosthodontics, Faculty of Dentistry, Trakya University, Istanbul, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Istanbul University, Istanbul, Turkey.
J Prosthodont. 2018 Jul;27(6):577-583. doi: 10.1111/jopr.12539. Epub 2016 Sep 6.
To evaluate the antimicrobial efficiency of three cleaning solutions and their effect on the physical properties of a denture base material.
A heat-cured polymethyl-methacrylate (PMMA) denture base material (Meliodent) and three cleaning solutions (alkaline-peroxide, 30 minutes; 1% sodium-hypochlorite, 10 minutes; and 0.1% polymeric-guanidine solution, 5 minutes) were used. For antifungal activity test, 40 disc-shaped specimens were fabricated and allocated into a control group (distilled water) and 3 experimental groups (n = 10) according to the cleaning solutions. Antifungal activity against Candida albicans (ATCC 2091) was assessed with colony-forming units. An additional 40 rectangular plate specimens were fabricated for mechanical tests. Ten specimens were kept intact to be used as the control group for flexural strength test. The remaining 30 specimens were distributed into three groups according to the cleaning solutions. The surface roughness and Vickers hardness of the specimens were consecutively measured after 48 hours of water storage at 37 ± 2°C (t0), two disinfection cycles (t1), and 7 days of storage (t2) in one of the solutions. Finally, all 40 rectangular specimens were subjected to flexural strength test. Data were analyzed with Kruskal-Wallis test for antifungal activity, ANOVA for flexural strength test, and analysis of covariance for surface roughness and hardness tests. Significance was set at 0.05.
The antifungal activities of polymeric guanidine and sodium hypochlorite were comparable to each other and significantly higher than alkaline peroxide (p < 0.05). The changes in the surface roughness of the specimens were statistically comparable among the cleaning solutions and time periods (p > 0.05); however, the decrease in the Vickers hardness of the specimens stored in sodium hypochlorite was significantly higher from t0 to t1 and t0 to t2 (p < 0.05) than other groups, resulting in comparable hardness changes. The flexural strengths of all groups were comparable with the control after t2 (p > 0.05).
The use of polymeric guanidine disinfectant solution could be an alternative method for cleaning PMMA denture base materials.
评估三种清洁溶液的抗菌效率及其对义齿基托材料物理性能的影响。
使用热固化聚甲基丙烯酸甲酯（PMMA）义齿基托材料（Meliodent）和三种清洁溶液（碱性过氧化物，30 分钟；1%次氯酸钠，10 分钟；0.1%聚合胍溶液，5 分钟）。对于抗真菌活性测试，制备了 40 个圆盘状试件，并根据清洁溶液将其分配到对照组（蒸馏水）和 3 个实验组（n=10）中。用菌落形成单位评估抗白色念珠菌（ATCC 2091）的抗真菌活性。另外制备了 40 个矩形板试件进行机械测试。10 个试件保持完整，用作弯曲强度测试的对照组。其余 30 个试件根据清洁溶液分为三组。将试件在 37±2°C 的水中储存 48 小时（t0）、两次消毒循环（t1）和 7 天（t2）后，分别连续测量试件的表面粗糙度和维氏硬度。最后，所有 40 个矩形试件均进行弯曲强度测试。用 Kruskal-Wallis 检验分析抗真菌活性，用方差分析（ANOVA）分析弯曲强度测试，用协方差分析测试表面粗糙度和硬度测试。显著性水平设定为 0.05。
聚合胍和次氯酸钠的抗真菌活性彼此相当，明显高于过氧化物碱性溶液（p<0.05）。各清洁溶液和时间段的试件表面粗糙度变化无统计学差异（p>0.05）；然而，与其他组相比，储存在次氯酸钠中的试件的维氏硬度在 t0 到 t1 和 t0 到 t2 时下降明显更高（p<0.05），导致硬度变化相当。所有组在 t2 后的弯曲强度与对照组相当（p>0.05）。
使用聚合胍消毒剂溶液可能是清洁 PMMA 义齿基托材料的替代方法。
