Hrdlicka J
Státni veterinární ústav, Jihlava.
Vet Med (Praha). 1990 Jul;35(7):411-8.
Three microbiological methods of agar diffusion were compared which are used to detect the residues of inhibitory substances: method using the strain Bacillus subtilis ATCC 6633 (B.s. 6633), method using the strain Bacillus stearothermophilus v. calidolactis C 953 (B. s. v. c. 953), and four-plate method. Using the compared methods, minimum inhibitory concentrations were determined for standard solutions of antibiotics and sulphadimidine. Inhibitory substances were detected parallely in the samples of the tissues of medicated and emergency-slaughtered animals, applying the three compared methods. In indicated cases inhibitory substances were identified by electrophoresis and chemical methods. The results indicate the following: 1) The B.s. 6633 method is the least sensitive of all to the residues of inhibitory substances. 2) For the purposes of detection of most antibiotics under investigation, the B.s.v.c. 953 method and the four-plate method are replaceable. The B.s.v.c. 953 method is more suitable for the detection of penicillin residues, in the case of tetracycline antibiotics it is the four-plate method. 3) Sulphadimidine residues can be detected only by the four-plate method. 4) The four-plate method enables to make the preliminary group identification of antibiotics and sulphonamides. 5) The detection limit of microbiological methods is not sufficiently sensitive to determine chloramphenicol residues; that is why the physico-chemical methods must be used.
使用枯草芽孢杆菌ATCC 6633菌株（B.s. 6633）的方法、使用嗜热脂肪芽孢杆菌嗜热栖热亚种C 953菌株（B. s. v. c. 953）的方法以及四平板法。使用这些比较的方法，测定了抗生素和磺胺嘧啶标准溶液的最低抑菌浓度。应用这三种比较的方法，同时检测了用药和紧急屠宰动物组织样本中的抑制性物质。在所示情况下，通过电泳和化学方法鉴定抑制性物质。结果表明：1）B.s. 6633方法对抑制性物质残留的敏感性在所有方法中最低。2）对于所研究的大多数抗生素的检测，B.s.v.c. 953方法和四平板法是可替代的。B.s.v.c. 953方法更适合检测青霉素残留，对于四环素类抗生素则是四平板法。3）磺胺嘧啶残留只能通过四平板法检测。4）四平板法能够对抗生素和磺胺类药物进行初步的分组鉴定。5）微生物学方法的检测限对氯霉素残留的测定不够敏感；因此必须使用物理化学方法。
