Koenen-Dierick K, Okerman L, de Zutter L, Degroodt J M, van Hoof J, Srebrnik S
Ministry of Public Health, Institute of Hygiene and Epidemiology, Brussels, Belgium.
Food Addit Contam. 1995 Jan-Feb;12(1):77-82. doi: 10.1080/02652039509374281.
A one-plate screening method is described for the microbiological detection of antibiotic residues by growth inhibition of Bacillus subtilis in agar medium, at pH 7, which contains trimethoprim for better detection of sulphonamides. beta-Glucuronidase is added to the samples to enable the detection of chloramphenicol residues. The sensitivity was determined for 16 antimicrobial substances frequently used in animal husbandry. A comparison was made with the sensitivity of the EEC four-plate test and existing maximum residue levels. The method has been used in Belgium for 5 years for the monitoring of antimicrobial residues in kidney tissue and meat of slaughter animals.
本文描述了一种单平板筛选方法，用于通过在pH值为7的琼脂培养基中抑制枯草芽孢杆菌的生长来微生物学检测抗生素残留，该培养基含有甲氧苄啶以更好地检测磺胺类药物。向样品中添加β-葡萄糖醛酸酶以检测氯霉素残留。测定了16种畜牧业常用抗菌物质的灵敏度。并与欧洲经济共同体的四平板试验灵敏度和现有最大残留限量进行了比较。该方法已在比利时用于监测屠宰动物肾脏组织和肉类中的抗菌残留5年。
