Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, IR Iran.
Phytother Res. 2010 Oct;24(10):1526-31. doi: 10.1002/ptr.3168.
Angiogenesis is a key process in the promotion of cancer and its metastasis. Herein, the antiangiogenic activity of Salvia officinalis extract and its fractions was investigated. S. officinalis aerial parts were extracted with ethanol and its successive hexane, ethyl acetate, n-butanol and aqueous fractions were evaluated for their antiangiogenic activities using human umbilical vein endothelial cells (HUVEC) capillary tube formation and rat aorta models in a three-dimensional collagen matrix. Furthermore, antimigrative effects of the fractions were assessed using a wound healing model. The ethanol extract of S. officinalis (ESO) potently inhibited capillary tube formation in HUVEC and rat aorta models of angiogenesis, and its hexane fraction (HSO) exerted the highest inhibitory effect. In addition, the ethanol extract of S. officinalis and its hexane fraction showed a dose-dependent inhibitory activity on the migration of the endothelial cells in the wound healing model. Furthermore, ESO inhibited endothelial cell proliferation at 50-200 μg/mL in a dose-dependent manner. These findings indicated some new pharmacological activities of S. officinalis such as antiangiogenic in vitro and ex vivo, and antimigrative activity in vitro. Therefore, S. officinalis could be a candidate as a useful herb with therapeutic or preventive activity against angiogenesis related disorders.
血管生成是促进癌症及其转移的关键过程。在此，研究了鼠尾草提取物及其馏分的抗血管生成活性。用乙醇提取鼠尾草地上部分，并用其连续的正己烷、乙酸乙酯、正丁醇和水馏分，用人脐静脉内皮细胞（HUVEC）毛细血管形成和大鼠主动脉在三维胶原基质中的模型来评估其抗血管生成活性。此外，还使用伤口愈合模型评估了馏分的抗迁移作用。鼠尾草乙醇提取物（ESO）强烈抑制 HUVEC 和血管生成大鼠主动脉模型中的毛细血管形成，其正己烷馏分（HSO）表现出最高的抑制作用。此外，鼠尾草乙醇提取物及其正己烷馏分在伤口愈合模型中对内皮细胞的迁移表现出剂量依赖性抑制活性。此外，ESO 以 50-200μg/mL 的剂量依赖性方式抑制内皮细胞增殖。这些发现表明鼠尾草具有一些新的药理学活性，如体外和体外的抗血管生成作用，以及体外的抗迁移活性。因此，鼠尾草可能是一种有治疗或预防与血管生成相关疾病的有用草药的候选物。
