Department of Materials Science and Engineering, National University of Singapore, 119260, Singapore.
J Am Chem Soc. 2010 Oct 27;132(42):14803-11. doi: 10.1021/ja103738t.
In the present study, quantum dot (QD) capped magnetite nanorings (NRs) with a high luminescence and magnetic vortex core have been successfully developed as a new class of magnetic-fluorescent nanoprobe. Through electrostatic interaction, cationic polyethylenimine (PEI) capped QD have been firmly graft into negatively charged magnetite NRs modified with citric acid on the surface. The obtained biocompatible multicolor QD capped magnetite NRs exhibit a much stronger magnetic resonance (MR) T2* effect where the r2* relaxivity and r2*/r1 ratio are 4 times and 110 times respectively larger than those of a commercial superparamagnetic iron oxide. The multiphoton fluorescence imaging and cell uptake of QD capped magnetite NRs are also demonstrated using MGH bladder cancer cells. In particular, these QD capped magnetite NRs can escape from endosomes and be released into the cytoplasm. The obtained results from these exploratory experiments suggest that the cell-penetrating QD capped magnetite NRs could be an excellent dual-modality nanoprobe for intracellular imaging and therapeutic applications. This work has shown great potential of the magnetic vortex core based multifunctional nanoparticle as a high performance nanoprobe for biomedical applications.
在本研究中，成功开发了具有高光致发光和磁性涡旋核的量子点（QD）包覆磁铁矿纳米环（NR），作为一类新型的磁性荧光纳米探针。通过静电相互作用，将带正电荷的聚乙烯亚胺（PEI）包覆的 QD 牢固地接枝到表面用柠檬酸修饰的带负电荷的磁铁矿 NR 上。所得的生物相容性多色 QD 包覆磁铁矿 NR 表现出更强的磁共振（MR）T2效应，其中 r2弛豫率和 r2*/r1 比值分别比商用超顺磁性氧化铁大 4 倍和 110 倍。还使用 MGH 膀胱癌细胞证明了 QD 包覆磁铁矿 NR 的多光子荧光成像和细胞摄取。特别是，这些 QD 包覆的磁铁矿 NR 可以从内涵体逃逸并释放到细胞质中。这些探索性实验的结果表明，穿透细胞的 QD 包覆的磁铁矿 NR 可以作为用于细胞内成像和治疗应用的优异的双模式纳米探针。这项工作表明，基于磁性涡旋核的多功能纳米颗粒作为用于生物医学应用的高性能纳米探针具有巨大的潜力。
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