Fricke H, Offen D, Mendlovic S, Shoenfeld Y, Bakimer R, Sperling J, Mozes E
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Int Immunol. 1990;2(3):225-30. doi: 10.1093/intimm/2.3.225.
Experimental systemic lupus erythematosus (SLE) in mice can be induced by immunization either with a human monoclonal anti-DNA antibody bearing the 16/6 idiotype (16/6 Id) or with a mouse monoclonal anti-idiotypic antibody specific for the 16/6 Id. In the present report we investigated the pathogenic role of a monoclonal anti-La autoantibody in the induction and mediation of experimental SLE in mice. The monoclonal anti-La antibody was derived from a mouse in which experimental SLE was induced by immunization with the monoclonal anti-16/6 Id antibody. Following immunization with the anti-La antibody the mice produced antibodies to double-stranded DNA, single-stranded DNA, Sm, SS-A/Ro, SS-B/La, and ribonucleoprotein. Furthermore, even though the anti-La antibody does not express nor react with the 16/6 Id, the immunized mice produced high titers of anti-16/6 Id antibodies as well as 16/6 Id bearing antibodies. Four months following immunization the mice exhibited significant proteinuria, and kidney sections revealed immune complex deposits on the basement membrane of the glomeruli. These results suggest that anti-La autoantibodies are involved in the induction and mediation of SLE in mice.
实验性系统性红斑狼疮（SLE）在小鼠中可通过用携带16/6独特型（16/6 Id）的人单克隆抗DNA抗体或用对16/6 Id特异的小鼠单克隆抗独特型抗体进行免疫来诱导。在本报告中，我们研究了一种单克隆抗La自身抗体在小鼠实验性SLE的诱导和介导中的致病作用。该单克隆抗La抗体源自一只通过用单克隆抗16/6 Id抗体免疫而诱导出实验性SLE的小鼠。在用抗La抗体免疫后，小鼠产生了针对双链DNA、单链DNA、Sm、SS - A/Ro、SS - B/La和核糖核蛋白的抗体。此外，尽管抗La抗体不表达也不与16/6 Id反应，但免疫后的小鼠产生了高滴度的抗16/6 Id抗体以及携带16/6 Id的抗体。免疫四个月后，小鼠出现明显的蛋白尿，肾脏切片显示肾小球基底膜上有免疫复合物沉积。这些结果表明抗La自身抗体参与了小鼠SLE的诱导和介导。
