用于高分辨率电子晶体学数据收集的膜蛋白二维晶体的制备。
Preparation of 2D crystals of membrane proteins for high-resolution electron crystallography data collection.
作者信息
Abeyrathne Priyanka D, Chami Mohamed, Pantelic Radosav S, Goldie Kenneth N, Stahlberg Henning
机构信息
C-CINA, Biozentrum, University of Basel, Basel, Switzerland.
出版信息
Methods Enzymol. 2010;481:25-43. doi: 10.1016/S0076-6879(10)81001-8.
Electron crystallography is a powerful technique for the structure determination of membrane proteins as well as soluble proteins. Sample preparation for 2D membrane protein crystals is a crucial step, as proteins have to be prepared for electron microscopy at close to native conditions. In this review, we discuss the factors of sample preparation that are key to elucidating the atomic structure of membrane proteins using electron crystallography.
电子晶体学是一种用于确定膜蛋白以及可溶性蛋白结构的强大技术。二维膜蛋白晶体的样品制备是关键步骤，因为蛋白质必须在接近天然条件下为电子显微镜进行制备。在本综述中，我们讨论了使用电子晶体学阐明膜蛋白原子结构的关键样品制备因素。
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