Nannenga Brent L, Gonen Tamir
Chemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, Tempe, AZ 85287, USA.
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Curr Opin Struct Biol. 2016 Oct;40:128-135. doi: 10.1016/j.sbi.2016.09.007. Epub 2016 Oct 1.
In 2013 we unveiled the cryo-electron microscopy (CryoEM) method of MicroED, or three-dimensional (3D) electron diffraction of microscopic crystals. Here tiny 3D crystals of biological material are used in an electron microscope for diffraction data collection under cryogenic conditions. The data is indexed, integrated, merged and scaled using standard X-ray crystallography software to determine structures at atomic resolution. In this review we provide an overview of the MicroED method and compare it with other CryoEM methods.
2013年，我们推出了微电子衍射（MicroED）的冷冻电子显微镜（CryoEM）方法，即微观晶体的三维（3D）电子衍射。在这里，生物材料的微小3D晶体被用于电子显微镜中，在低温条件下收集衍射数据。使用标准的X射线晶体学软件对数据进行索引、整合、合并和缩放，以确定原子分辨率的结构。在这篇综述中，我们概述了MicroED方法，并将其与其他CryoEM方法进行了比较。
