微晶电子衍射开启了生物结构测定的新纪元。
MicroED opens a new era for biological structure determination.
作者信息
Nannenga Brent L, Gonen Tamir
机构信息
Chemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, Tempe, AZ 85287, USA.
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
出版信息
Curr Opin Struct Biol. 2016 Oct;40:128-135. doi: 10.1016/j.sbi.2016.09.007. Epub 2016 Oct 1.
Abstract
In 2013 we unveiled the cryo-electron microscopy (CryoEM) method of MicroED, or three-dimensional (3D) electron diffraction of microscopic crystals. Here tiny 3D crystals of biological material are used in an electron microscope for diffraction data collection under cryogenic conditions. The data is indexed, integrated, merged and scaled using standard X-ray crystallography software to determine structures at atomic resolution. In this review we provide an overview of the MicroED method and compare it with other CryoEM methods.
摘要
2013年，我们推出了微电子衍射（MicroED）的冷冻电子显微镜（CryoEM）方法，即微观晶体的三维（3D）电子衍射。在这里，生物材料的微小3D晶体被用于电子显微镜中，在低温条件下收集衍射数据。使用标准的X射线晶体学软件对数据进行索引、整合、合并和缩放，以确定原子分辨率的结构。在这篇综述中，我们概述了MicroED方法，并将其与其他CryoEM方法进行了比较。
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本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED.通过微电子衍射从朊病毒纳米晶体中以原子分辨率从头确定结构。
Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11232-11236. doi: 10.1073/pnas.1606287113. Epub 2016 Sep 19.
3
Modeling truncated pixel values of faint reflections in MicroED images.对MicroED图像中微弱反射的截断像素值进行建模。
J Appl Crystallogr. 2016 May 11;49(Pt 3):1029-1034. doi: 10.1107/S1600576716007196. eCollection 2016 Jun 1.
4
The collection of MicroED data for macromolecular crystallography.用于大分子晶体学的微晶电子衍射（MicroED）数据收集
Nat Protoc. 2016 May;11(5):895-904. doi: 10.1038/nprot.2016.046. Epub 2016 Apr 14.
5
Structure of the toxic core of α-synuclein from invisible crystals.来自不可见晶体的α-突触核蛋白毒性核心结构。
Nature. 2015 Sep 24;525(7570):486-90. doi: 10.1038/nature15368. Epub 2015 Sep 9.
6
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7
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Science. 2015 Jun 5;348(6239):1147-51. doi: 10.1126/science.aab1576. Epub 2015 May 7.
8
Electron crystallography of ultrathin 3D protein crystals: atomic model with charges.超薄三维蛋白质晶体的电子晶体学：带电荷的原子模型
Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3368-73. doi: 10.1073/pnas.1500724112. Epub 2015 Feb 17.
9
Precession electron diffraction - a topical review.进动电子衍射——一篇专题综述。
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10
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Elife. 2014 Oct 10;3:e03600. doi: 10.7554/eLife.03600.
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