Department of Chemistry, University of Crete, Heraklion, Crete, Greece.
Int Microbiol. 2010 Sep;13(3):143-50. doi: 10.2436/20.1501.01.119.
The variability of bacterial community composition and diversity was studied by comparative analysis of five 16S rRNA gene clone libraries from deep-sea sediments (water column depth: 4000 m) of the Northeastern Pacific Ocean and Eastern Mediterranean Sea. This is the first comparison of the bacterial communities living in these deep-sea ecosystems. The estimated chlorophyll a, organic carbon, and C/N ratio provided evidence of significant differences in the trophic state of the sediments between the Northeastern Pacific Ocean and the much warmer Eastern Mediterranean Sea. A diverse range of 16S rRNA gene phylotypes was found in the sediments of both regions. These were represented by 11 different taxonomic groups, with Gammaproteobacteria predominating in the Northeastern Pacific Ocean sediments and Acidobacteria in the Eastern Mediterranean microbial community. In addition, several 16S rRNA gene phylotypes only distantly related to any of the previously identified sequences (non-affiliated rRNA genes) represented a significant fraction of the total sequences. The potential diversity at the two sites differs but remains largely unexplored and remains of continuing scientific interest.
通过对东北太平洋和东地中海深海沉积物（水柱深度：4000 米）的 5 个 16S rRNA 基因克隆文库进行比较分析，研究了细菌群落组成和多样性的可变性。这是对生活在这些深海生态系统中的细菌群落的首次比较。估计的叶绿素 a、有机碳和 C/N 比为东北太平洋和温暖得多的东地中海沉积物之间的营养状态差异提供了证据。在两个地区的沉积物中发现了广泛的 16S rRNA 基因系统发育型。这些系统发育型代表了 11 个不同的分类群，其中γ变形菌在东北太平洋沉积物中占优势，而东地中海微生物群落中的酸杆菌占优势。此外，一些与之前鉴定的任何序列（非相关 rRNA 基因）仅有远缘关系的 16S rRNA 基因系统发育型代表了总序列的重要部分。两个地点的潜在多样性不同，但仍在很大程度上未被探索，仍然是科学关注的持续热点。
