丝氨酸/苏氨酸蛋白磷酸酶测定
Serine/threonine protein phosphatase assays.
作者信息
McAvoy Thomas, Nairn Angus C
机构信息
The Rockefeller University, New York, New York, USA.
出版信息
Curr Protoc Mol Biol. 2010 Oct;Chapter 18:Unit18.18. doi: 10.1002/0471142727.mb1818s92.
Abstract
Methods for assaying serine/threonine protein phosphatases are discussed. Three commonly used protocols are presented that employ either colorimetric or radiometric assays. These methods can be used to assay a variety of preparations of serine/threonine phosphatases, from crude lysates to purified proteins. Strategies for the application of a particular protocol for a particular purpose are discussed. The assays can be used in either a high-throughput mode where simple comparison of activities can be done, or in specific assays where kinetic data can be derived.
摘要
本文讨论了测定丝氨酸/苏氨酸蛋白磷酸酶的方法。介绍了三种常用的方案，这些方案采用比色法或放射性测定法。这些方法可用于测定从粗裂解物到纯化蛋白的各种丝氨酸/苏氨酸磷酸酶制剂。讨论了针对特定目的应用特定方案的策略。这些测定法可用于高通量模式，在此模式下可进行简单的活性比较，也可用于可得出动力学数据的特定测定中。
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