Zingler G, Schmidt G, Orskov I, Orskov F, Falkenhagen U, Naumann G
Institut für Medizinische Mikrobiologie, Universität Rostock.
Zentralbl Bakteriol. 1990 Dec;274(3):372-81. doi: 10.1016/s0934-8840(11)80695-x.
Serotyping of 1918 Escherichia coli strains isolated in significant cell numbers from the urine of patients with urinary tract infections (UTI) revealed the presence of 117 O6 strains. The K antigens were identified by means of K-specific phages and serological methods. The phages used included a K1 phage pool (phi 1, A-E) and the separate phages phi 2, phi 5, phi 7, phi 12 and phi 13. The presence of H antigens, type 1 fimbriae formation, hemolysin production and mannose-resistant hemagglutination (MRHA) ability with human A, sheep, calf and pig erythrocytes were also analyzed. Six different MRHA types were defined and discussed in relation to the O6:K:H serotype. Remarkably, E. coli O6 strains were found to possess a whole arsenal of virulence factors (K antigens, MRHA, hemolysin). The most common serotypes - O6:K2:H1/H- (26), O6:K5:H1/H- (35) and O6:K13:H1/H- (20) - differed from each other in some cases in both MRHA type and hemolysin production.
从尿路感染（UTI）患者尿液中大量分离出的1918株大肠杆菌进行血清型鉴定，结果显示有117株O6菌株。通过K特异性噬菌体和血清学方法鉴定K抗原。所用噬菌体包括一个K1噬菌体库（phi 1，A - E）以及单独的噬菌体phi 2、phi 5、phi 7、phi 12和phi 13。还分析了H抗原的存在、1型菌毛的形成、溶血素的产生以及对人A、绵羊、小牛和猪红细胞的甘露糖抗性血凝（MRHA）能力。定义了六种不同的MRHA类型，并结合O6:K:H血清型进行了讨论。值得注意的是，发现大肠杆菌O6菌株具有一系列毒力因子（K抗原、MRHA、溶血素）。最常见的血清型——O6:K2:H1/H-（26株）、O6:K5:H1/H-（35株）和O6:K13:H1/H-（20株）——在某些情况下，MRHA类型和溶血素产生情况彼此不同。
