用酸性乙醇提取后植物化诱导因子的分离。该因子的浓度依赖性诱导能力。
Isolation of a vegetalizing inducing factor after extraction with acid ethanol. Concentration-dependent inducing capacity of the factor.
作者信息
Plessow S, Davids M, Born J, Hoppe P, Loppnow-Blinde B, Tiedemann H, Tiedemann H
机构信息
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, F.R.G.
出版信息
Cell Differ Dev. 1990 Oct;32(1):27-38. doi: 10.1016/0922-3371(90)90096-f.
A vegetalizing factor has been isolated from chicken embryos by an improved method. The factor is extracted with acid/ethanol and finally purified by four consecutive steps of reversed phase HPLC. The molecular mass is about 25 kDa. The factor dissociates after reduction with dithiothreitol into two subunits of about 13 kDa. The factor was tested on Triturus alpestris by the implantation method, and on isolated ectoderm of Xenopus laevis in solution. The factor induces as the crude fractions all types of mesodermal tissues dependent on the concentration of the factor.
通过一种改进的方法从鸡胚中分离出了一种促使组织向植物性组织分化的因子。该因子用酸/乙醇提取，最后通过反相高效液相色谱的四个连续步骤进行纯化。其分子量约为25 kDa。用二硫苏糖醇还原后，该因子解离成两个约13 kDa的亚基。通过植入法在高山蝾螈上以及在溶液中对非洲爪蟾的离体外胚层进行了该因子的测试。该因子作为粗提物，根据其浓度诱导出所有类型的中胚层组织。
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