鸡胚胎中的植物化因子：其类EDF（激活素A）活性。

The vegetalizing factor from chicken embryos: its EDF (activin A)-like activity.

作者信息

Asashima M, Uchiyama H, Nakano H, Eto Y, Ejima D, Sugino H, Davids M, Plessow S, Born J, Hoppe P

机构信息

Department of Biology, Yokohama City University, Japan.

出版信息

Mech Dev. 1991 Jun;34(2-3):135-41. doi: 10.1016/0925-4773(91)90050-g.

DOI:10.1016/0925-4773(91)90050-g

PMID:1911396

Abstract

The erythroid differentiation capacity of the HPLC-purified mesoderm- and endoderm-inducing vegetalizing factor from chicken embryos and of recombinant erythroid differentiation factor (EDF = activin A), an evolutionary highly conserved member of the TGF-beta protein superfamily have been compared. Both factors stimulate the synthesis of hemoglobin in erythroleukemia cells in the same concentration range. The EDF-activity of the mesoderm-inducing HPLC-fractions is inhibited by follistatin, an EDF-binding protein. The factor induces in ectoderm of Triturus taeniatus all kinds of mesodermal organs. The wide spectrum of organs is very likely to be induced by secondary interactions. At higher concentration (15 ng/ml), notochord- and endoderm-like tissues are induced in a high percentage.

摘要

对从鸡胚中经高效液相色谱法纯化的中胚层和内胚层诱导植物化因子以及重组红细胞分化因子（EDF = 激活素A，转化生长因子-β蛋白超家族中进化上高度保守的成员）的红细胞分化能力进行了比较。两种因子在相同浓度范围内均能刺激红白血病细胞中血红蛋白的合成。中胚层诱导高效液相色谱级分的EDF活性受到一种EDF结合蛋白——卵泡抑素的抑制。该因子能在带状蝾螈的外胚层诱导出各种中胚层器官。如此广泛的器官种类很可能是由次级相互作用诱导产生的。在较高浓度（15纳克/毫升）时，能诱导出高比例的脊索样和内胚层样组织。