Bass D A, Dechatelet L R, McCall C E
J Immunol. 1978 Jul;121(1):172-8.
It has recently been suggested that a single stimulus to the membrane of the polymorphonuclear neutrophil leukocyte (PMN) produces a sequential, stereotyped response involving motility, degranulation, and the oxidative metabolic burst, and conversely, that the chemotactic response is dependent upon the stimulation of the hexose monophosphate shunt (HMPS). We have used small, synthetic substances, known to cause either increased motility or the metabolic burst, to examine whether these events can be stimulated independently. Phorbol myristate acetate (PMA) is a surface active agent that causes marked stimulation of iodination, superoxide production, chemiluminescence, and the HMPS. Such stimulation by PMA did not alter random or directional motility of PMN in the chemotaxis-under-agarose assay. Also, preincubation of PMN with PMA did not deplete their energy source for chemotaxis as demonstrated by a normal chemotactic response to zymosan activated serum. N-formylmethionyl peptides (f-met-phe, f-met-leu-phe) caused a dose-related stimulation of random and directional motility of PMN, but only a very slight stimulation of the HMPS, protein iodination, superoxide production, or chemiluminescence, and this minimal response occurred at more than 1000 times the concentration needed for stimulation of motility. These results indicate that stimulation of motility in the metabolic burst may involve separate events at the membrane of the PMN and that the events are not necessarily interdependent.
最近有人提出，对多形核嗜中性白细胞（PMN）细胞膜的单次刺激会产生一系列刻板的反应，包括运动、脱颗粒和氧化代谢爆发，反之，趋化反应依赖于磷酸己糖旁路（HMPS）的刺激。我们使用了已知会引起运动增加或代谢爆发的小分子合成物质，来研究这些事件是否可以独立刺激。佛波酯（PMA）是一种表面活性剂，可显著刺激碘化、超氧化物产生、化学发光和HMPS。PMA的这种刺激在琼脂糖趋化试验中并未改变PMN的随机或定向运动。此外，用PMA预孵育PMN并没有耗尽它们用于趋化的能量来源，这通过对酵母聚糖激活血清的正常趋化反应得到证明。N-甲酰甲硫氨酰肽（f-met-phe、f-met-leu-phe）引起了PMN随机和定向运动的剂量相关刺激，但对HMPS、蛋白质碘化、超氧化物产生或化学发光只有非常轻微的刺激，而且这种最小反应发生时的浓度是刺激运动所需浓度的1000倍以上。这些结果表明，刺激PMN的运动和代谢爆发可能涉及细胞膜上的不同事件，而且这些事件不一定相互依赖。
