Biotechnology Research Centre, La Trobe University, Edwards Road, Bendigo, Victoria, Australia.
Crit Rev Biotechnol. 2011 Jun;31(2):170-85. doi: 10.3109/07388551.2010.505909. Epub 2010 Oct 5.
There is considerable interest in exploiting the novel physical and biological properties of microbial exopolysaccharides in industry and medicine. For economic and scientific reasons, large scale production under carefully monitored and controlled conditions is required. Producing exopolysaccharides in industrial fermenters poses several complex bioengineering and microbiological challenges relating primarily to the very high viscosities of such culture media, which are often exacerbated by the producing organism's morphology. What these problems are, and the strategies for dealing with them are discussed critically in this review, using pullulan, curdlan, xanthan, and fungal β-glucans as examples of industrially produced microbial exopolysaccharides. The role of fermenter configuration in their production is also examined.
人们对利用微生物胞外多糖的新颖物理和生物特性在工业和医学中的应用非常感兴趣。出于经济和科学原因，需要在精心监控和控制条件下进行大规模生产。在工业发酵罐中生产胞外多糖主要存在一些复杂的生物工程和微生物学挑战，这些挑战主要与这些培养基的极高粘度有关，而产生菌的形态往往会使问题更加严重。本文以普鲁兰、卡拉胶、黄原胶和真菌β-葡聚糖为例，对这些问题以及应对策略进行了批判性讨论。本文还考察了发酵罐结构在其生产中的作用。
