Department of Medical Chemistry and Biochemistry, 2nd Faculty of Medicine Charles Universtity in Prague, Czech Republic.
Exp Gerontol. 2011 Jan;46(1):38-42. doi: 10.1016/j.exger.2010.09.015. Epub 2010 Oct 12.
Oxidative stress, which is present in Alzheimer's disease (AD), results in the formation of various end-products of free radical reactions with proteins and lipids. At present there are no reliable diagnostic biomarkers of AD in the blood. Therefore, specific products of lipid peroxidation in the blood of AD patients were investigated. Lipophilic extracts of erythrocytes in the group of patients with AD (n = 44) and age-matched controls (n = 16) were studied. The end-products of lipid peroxidation, so called lipofuscin-like pigments (LFP), were analysed by fluorescence spectroscopy. It was found that the level of these products is significantly increased in erythrocytes of AD patients compared to controls. LFP were further separated by means of HPLC into individual fractions to study their composition in AD and controls. The specific fraction of LFP in AD patients, which was isolated, might represent a disease-specific product in the blood.
氧化应激存在于阿尔茨海默病（AD）中，导致蛋白质和脂质与自由基反应的各种终产物的形成。目前，血液中尚无可靠的 AD 诊断生物标志物。因此，研究了 AD 患者血液中脂质过氧化的特定产物。研究了 AD 患者组（n = 44）和年龄匹配的对照组（n = 16）的红细胞脂溶性提取物。通过荧光光谱法分析脂质过氧化的终产物，即类脂褐素样色素（LFP）。结果发现，与对照组相比，AD 患者红细胞中这些产物的水平显著升高。通过 HPLC 将 LFP 进一步分离成单独的馏分，以研究它们在 AD 和对照组中的组成。从 AD 患者中分离出的特定 LFP 馏分可能代表血液中的疾病特异性产物。
