Serbetçi Tuba, Demirci Betül, Güzel Cağla Bozkurt, Kültür Sükran, Ergüven Mine, Başer Kemal Hüsnü Can
Department of Pharmacognosy, Faculty of Pharmacy, Istanbul University, 34116 Istanbul, Turkey.
Nat Prod Commun. 2010 Sep;5(9):1369-74.
The chemical compositions of the water-distilled essential oil of Stachys cretica ssp. lesbiaca Rech. fil. and S. cretica ssp. trapezuntica Rech. fil. were determined by GC and GC-MS. Altogether 63 compounds were identified. The sesquiterpene hydrocarbon, germacrene D (20.3% and 12.9% respectively) was the main component identified in both oils. Furthermore, ethanol, light petroleum, dichloromethane, ethyl acetate and n-butanol extracts prepared from the aerial parts of the plants were tested for their antimicrobial activities against six bacterial strains and the yeast Candida albicans. The extracts exhibited no antibacterial activity, but the light petroleum and n-butanolic fractions showed low antifungal activities. Crude ethanolic extracts of the two subspecies were tested for their ability to inhibit the growth of HL-60 and Ishikawa human tumor cell lines. The IC50 values were 100 microg/mL for the HL-60 cell line and 200 microg/mL for the Ishikawa cell line.
对克里特水苏亚种莱斯博斯水苏（Stachys cretica ssp. lesbiaca Rech. fil.）和克里特水苏亚种特拉佩祖恩特斯水苏（S. cretica ssp. trapezuntica Rech. fil.）的水蒸气蒸馏精油的化学成分进行了气相色谱（GC）和气相色谱 - 质谱联用（GC-MS）分析。共鉴定出63种化合物。倍半萜烃类，即杜松烯D（分别为20.3%和12.9%）是两种精油中鉴定出的主要成分。此外，还测试了从植物地上部分制备的乙醇、轻质石油、二氯甲烷、乙酸乙酯和正丁醇提取物对六种细菌菌株和白色念珠菌酵母的抗菌活性。提取物没有表现出抗菌活性，但轻质石油和正丁醇部分显示出较低的抗真菌活性。测试了这两个亚种的粗乙醇提取物抑制HL-60和石川人肿瘤细胞系生长的能力。HL-60细胞系的半数抑制浓度（IC50）值为100微克/毫升，石川细胞系的IC50值为200微克/毫升。
