Reizer J, Reizer A, Saier M H
Department of Biology, University of California at San Diego, La Jolla 92093-0116.
Res Microbiol. 1990 Nov-Dec;141(9):1061-7. doi: 10.1016/0923-2508(90)90079-6.
The cellobiose (cel) operon of Escherichia coli was recently sequenced and shown to consist of five genes, celABCDF (Parker and Hall, 1990). We have shown that the CelA, CelB and CelC proteins possess amino acid sequences which are homologous to different domains of the lactose permease of Staphylococcus aureus. CelB corresponds to the integral membrane portion of the permease (IIcel) while CelC (IIIcel) and CelA (IVcel) correspond to the two cytoplasmic domains which appear to comprise the first and second phosphorylation sites in the permease, respectively. The cellobiose permease is the only one of several homologous sequenced permeases of the phosphoenolpyruvate:sugar phosphotransferase system which has its three known functional domains residing on distinct polypeptide chains.
大肠杆菌的纤维二糖（cel）操纵子最近已被测序，结果显示它由五个基因celABCDF组成（帕克和霍尔，1990年）。我们已经表明，CelA、CelB和CelC蛋白的氨基酸序列与金黄色葡萄球菌乳糖通透酶的不同结构域具有同源性。CelB对应于通透酶的整合膜部分（IIcel），而CelC（IIIcel）和CelA（IVcel）分别对应于通透酶中似乎构成第一和第二磷酸化位点的两个细胞质结构域。纤维二糖通透酶是磷酸烯醇式丙酮酸：糖磷酸转移酶系统中几个同源测序通透酶中唯一的一个，其三个已知功能结构域位于不同的多肽链上。
