Ajinomoto-Genetika Research Institute, 1-st Dorozhny pr. 1-1, Moscow 117545, Russia.
Fitoterapia. 2011 Mar;82(2):212-8. doi: 10.1016/j.fitote.2010.09.013. Epub 2010 Oct 12.
An aqueous ethanol extract of Bergenia crassifolia rhizomes strongly inhibited human pancreatic lipase activity and increased scavenging of DPPH free radicals in vitro. Chromatographic separation of this extract led to isolation of the hydrolysable tannins (+)-catechin 3,5-di-O-gallate (1) and (+)-catechin 3-O-gallate (2). This is the first report of the isolation of compound 1 from plant material. This compound strongly inhibited human pancreatic lipase (with an IC(50) value of 0.42 μg/ml) and exhibited a remarkable free radical-scavenging ability (with an SC(50) value of 1.04 μg/ml). The chemical structures of 1 and 2 were elucidated using MS, NMR and chemical approaches.
宝兴玉兰根茎的水-乙醇提取物强烈抑制人胰腺脂肪酶的活性，并增加体外对 DPPH 自由基的清除作用。该提取物的色谱分离导致可水解鞣质（+）-儿茶素 3,5-二-O-没食子酸酯（1）和（+）-儿茶素 3-O-没食子酸酯（2）的分离。这是首次从植物材料中分离出化合物 1。该化合物强烈抑制人胰腺脂肪酶（IC50 值为 0.42μg/ml），并表现出显著的自由基清除能力（SC50 值为 1.04μg/ml）。通过 MS、NMR 和化学方法阐明了 1 和 2 的化学结构。
