Caruso Rosario Alberto, Fedele Francesco, Crisafulli Costantino, Paparo Domenica, Parisi Antonino, Lucianò Roberta, Cavallari Vittorio
Department of Human Pathology, University Hospital, Messina, Italy.
Ultrastruct Pathol. 2011 Feb;35(1):14-8. doi: 10.3109/01913123.2010.517899. Epub 2010 Oct 7.
The authors report a case of a 70-year-old woman with an anaplastic giant cell thyroid carcinoma, along with immunohistochemical and electron microscopic findings. Histologically, the tumor is characterized by mononucleated and multinucleated giant cells, lack of architectural cohesion, atypical mitoses, and extensive areas of coagulative necrosis. Tumor cells showed AE1/AE3 positivity as well as nuclear overexpression of p53 and ki-67. Semithin sections revealed multiple nuclei with heterogeneous size ranging from micronuclei to large-size (giant) nuclei. Micronuclei were confirmed by electron microscopy that disclosed also the presence of nuclear blebs, strings, and pockets. Morphological findings of these abnormal nuclear structures in conjunction with p53 and Ki-67 nuclear overexpression suggested a faulty mitotic checkpoint/mitotic catastrophe in the progression of anaplastic giant cell thyroid carcinoma.
作者报告了一例70岁女性的间变性巨细胞甲状腺癌病例，并给出了免疫组织化学和电子显微镜检查结果。组织学上，肿瘤的特征为单核和多核巨细胞、缺乏结构黏附性、非典型有丝分裂以及广泛的凝固性坏死区域。肿瘤细胞显示AE1/AE3阳性，同时p53和ki-67核过表达。半薄切片显示多个大小不一的核，范围从微核到大型（巨）核。电子显微镜证实了微核的存在，同时还发现了核泡、核索和核袋。这些异常核结构的形态学发现与p53和Ki-67核过表达相结合，提示在间变性巨细胞甲状腺癌进展过程中有丝分裂检查点/有丝分裂灾难存在缺陷。
