Department of Zoology, Ket's V. G. Vaze College, Mulund, Mumbai, India.
In Vitro Cell Dev Biol Anim. 2010 Dec;46(10):824-7. doi: 10.1007/s11626-010-9349-4. Epub 2010 Oct 7.
Poekilocerus pictus is a painted grasshopper, which feeds on Calotropis sp. containing the cardiac glycosides. A new cell line BPH22 is developed from midgut of P. pictus to study its unique physiology and biochemistry. Initially, the Graces insect medium is used with 10% (v/v) fetal bovine serum. After four passages, the serum quantity was reduced up to 0%.The lag phase in growth curve was 5 d and log phase is up to 10-11 d. The addition of Calotropis extract in Graces medium enhanced the growth of cells. The Calotropis extract with Graces medium altered the morphology of cells of BPH22. The amplification and sequencing of 16S rRNA gene confirmed the origin and purity of the BPH22.
波凯兜蝽是一种食叶型的昆虫，以含有强心苷的马利筋为食。为了研究其独特的生理学和生物化学性质，我们从波凯兜蝽的中肠开发了一种新的细胞系 BPH22。最初，我们使用 Grace's 昆虫培养基，并添加 10%（体积/体积）胎牛血清。经过 4 次传代后，血清量减少到 0%。生长曲线的迟滞期为 5 天，对数期长达 10-11 天。在 Grace's 培养基中添加马利筋提取物可促进细胞生长。马利筋提取物和 Grace's 培养基共同作用改变了 BPH22 细胞的形态。16S rRNA 基因的扩增和测序证实了 BPH22 的来源和纯度。
